Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization
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概要
- 論文の詳細を見る
An α-galactosidase gene belonging to glycoside hydrolase family 27 from Streptomyces coelicolor was cloned and expressed in E. coli. The purified enzyme showed a single protein band on SDS-PAGE with a molecular mass of 64kDa. It was quite stable from pH 5.0 to 10.0 after treatment at 40°C for 60min, and was thermally stable up to 50°C. The enzyme acted on galacto-oligosaccharides, galactomanno-oligosaccharides and galactomannans as well as plant α-galactosidases. It consisted of an N-terminal catalytic domain (400 amino acid residues) and a C-terminal region (260 amino acid residues). The catalytic domain of the enzyme was constructed by deleting the C-terminal region from the enzyme and was found to be stable from pH 5.5 to 8.5 and up to 40°C. The catalytic domain showed the same specificity towards galacto-oligosaccharides, galactomanno-oligosaccharides and galactomannans as the enzyme containing the C-terminal region. These results indicated that the Cterminal region probably has an important role in stabilizing the enzyme.
- 社団法人 日本食品科学工学会の論文
- 2005-05-01
著者
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Kobayashi H
National Food Research Institute
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KANEKO Satoshi
Food Biotechnology Division, National Food Research Institute
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Kaneko Satoshi
Food Biotechnology Div. National Food Res. Inst.
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Kobayashi Hideyuki
Molecular Function Laboratory, National Food Research Institute
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Kobayashi H
National Food Res. Inst. Ibaraki Jpn
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Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
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Morisaki Kohei
Institute Of Applied Biochemistry University Of Tsukuba
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Kaneko Satoshi
National Food Res. Inst.
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Kaneko S
Food Biotechnology Division National Food Research Institute
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KONDOH Kenji
Graduated School of Life and Environmental Science, University of Tsukuba
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MORISAKI Kohei
Graduated School of Life and Environmental Science, University of Tsukuba
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KIM Wook-Dong
Molecular Function Laboratory, National Food Research Institute
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KOTWAL Sahebral
National Collection of Industrial Microorganisms Division of Biochemical Science, Chemical Research
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KANEKO Satoshi
Molecular Function Laboratory, National Food Research Institute
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Kaneko Shigenobu
National Institute Of Crop Science (nics)
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Kaneko Satoshi
Food Biotechnology Division National Food Research Institute
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Kondoh Kenji
Graduated School Of Life And Environmental Science University Of Tsukuba
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Kim Wook-dong
Food Biotechnology Division National Food Research Institute National Agriculture And Food Research
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Kotwal Sahebral
National Collection Of Industrial Microorganisms Division Of Biochemical Science Chemical Research L
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