Purification and Characterization of the 2nd 5'-Nucleotide-forming Nuclease from Lentinus edodes
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概要
- 論文の詳細を見る
The 2nd endonuclease (nuclease Le_3) which hydrolyzes both RNA and heat denatured DNA to 5'-nucleotides was purified from the fruit bodies of Lentinus edodes to a homogeneous state by SDS PAGE. The nuclease was different from the nuclease Le_1 previously characterized [H. Shimada et al. Chem. Pharm. Bull., 39, 2633-2637 (1991)] in molecular weight, optimal pH and N-terminal amino acid sequence. The N-terminal amino acid sequence of nuclease Le_3analyzed up to the 20th residue showed that 50% of the amino acid residues are identical to nuclease Le_1.
- 社団法人日本農芸化学会の論文
- 1995-06-23
著者
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Koyama Takashi
Department of Cardiovascular and Respiratory Medicine, Akita University Graduate School of Medicine
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Koyama Takashi
Division Of Life Sciences Graduate School Of Natural Science And Technology Kanazawa University
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Irie Masachika
Department of Microbiology, Hoshi College of Pharmacy
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Irie Masachika
Department Of Applied Microbiology Faculty Of Pharmaceutical Sciences Hoshi University
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Irie Masachika
Department Of Microbiology Hoshi College Of Pharmacy
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Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
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KOBAYASHI Hiroko
Department of Internal Medicine II, Fukushima Medical University School of Medicine
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Koyama Takashi
Department Of Microbiology College Of Pharmacy Nihon University
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TOMITA Motowo
Department of Physiological Chemistry, School of Pharmaceutical Sciences Showa University
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INOKUCHI Norio
Department of Microbiology, College of Pharmacy, Nihon University
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Tomita M
Department Of Drug Absorption And Pharmacokinetics School Of Pharmacy Tokyo University Of Pharmacy A
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Tomita Motowo
Department Of Hygienic Chemistry School Of Pharmaceutical Sciences Showa University
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Irie M
Hoshi Coll. Pharmacy Tokyo Jpn
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Inokuchi N
Department Of Microbiology College Of Pharmacy Nihon University
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Kobayashi Hiroko
Department Of Gastroenterology And Rheumatology Fukushima Medical University School Of Medicine
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Koyama Takashi
Department of Cardiology, Akita University School of Medicine
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Kobayashi Hiroko
Department of Cardiovascular Disease Research, Eisai Tsukuba Research Laboratories
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