Development of a Gene Transfer System for the Mycelia of Flammulina velutipes Fv-1 Strain
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概要
- 論文の詳細を見る
To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enzyme mediated integration method.
- 2010-05-23
著者
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Yoshida Makoto
Department of Internal Medicine, Kitasato University School of Medicine
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TAKABATAKE Koji
Toyama Prefectural Agricultural, Forestry and Fisheries Research Center, Forest Research Institute
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KANEKO Satoshi
Food Biotechnology Division, National Food Research Institute
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Kaneko Satoshi
Food Biotechnology Div. National Food Res. Inst.
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Yoshida M
Molecular Photoscience Research Center Kobe University:institute For Solid State Physics University
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Ito Yasuhiro
Food Biotechnology Div. National Food Res. Inst.
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Kaneko Satoshi
National Food Res. Inst.
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Takabatake Koji
Toyama Prefectural Agricultural Forestry And Fisheries Research Center Forest Research Institute
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Kaneko Shigenobu
National Institute Of Crop Science (nics)
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Kaneko Satoshi
Food Biotechnology Division National Food Research Institute
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ICHINOSE Hitomi
Food Biotechnology Division, National Food Research Institute
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MAEHARA Tomoko
Food Biotechnology Division, National Food Research Institute
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TOMITA Shizuko
Food Biotechnology Division, National Food Research Institute
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TAKABATAKE Koji
Forest Institute, Toyama Prefectural Agricultural, Forestry, and Fisheries Research Center
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Yoshida Makoto
Faculty Of The Integated Arts And Sciences Hiroshima University
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Maehara Tomoko
Food Biotechnology Division National Food Research Institute
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Ichinose Hitomi
National Food Res. Inst.
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Tomita Shizuko
Food Biotechnology Division National Food Research Institute
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Yoshida Makoto
Department Of Clinical Pharmacology And Therapeutics Tohoku University Graduate School Of Pharmaceut
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Yoshida Makoto
Department Of Biology Faculty Of Science University Of Kochi
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Ito Yasuhiro
Food Biotechnology Div. National Food Res. Inst. National Agriculture And Food Res. Organization (na
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Yoshida Makoto
Department Of Environmental And Natural Resource Science Faculty Of Agriculture Tokyo University Of
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Yoshida Makoto
Fac. Of Agriculture Tokyo Univ. Of Agriculture And Technol.
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