Production and Characterization of Recombinant Phanerochaete chrysosporium β-Glucosidase in the Methylotrophic Yeast Pichia pastoris(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
The extracellular β-glucosidase from the white-rot fungus Phanerochaete chrysosporium was expressed heterologously in the methylotrophic yeast Pichia pastoris. After 7 days' cultivation in an induction medium containing 1% (v/v) methanol, the expression level of the recombinant enzyme was 28,500 U/I, 38 times that of the wild-type enzyme. The specific activity of the crude recombinant enzyme forp-nitrophenyl-β-D-glucoside was 52 U/mg, 37 times that of the wild-type enzyme ; this difference made the purification of the enzyme simple. On a SDS-PAGE, the molecular mass of the recombinant enzyme was 133 kDa, and that of the wild-type enzyme was 116 kDa, but the difference had no effect on the hydrolysis of cellobiose or p-nitrophenyl-β-D-glucoside. We concluded that the recombinant enzyme produced by Pichia pastoris retains the catalytic properties of the wild-type enzyme from Phanerochaete chrysosporium.
- 社団法人日本農芸化学会の論文
- 2003-01-23
著者
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IGARASHI Kiyohiko
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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SAMEJIMA Masahiro
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Nagasawa Hiromichi
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Ohira Tsuyoshi
Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology
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Ohira T
Kanagawa Univ. Kanagawa Jpn
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Ohira Tsuyoshi
Faculty Of Science Kanagawa University
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Ohira Tsuyoshi
Laboratory Of Genome Science Graduate School Of Marine Science And Technology Tokyo University Of Ma
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Yoshida M
Molecular Photoscience Research Center Kobe University:institute For Solid State Physics University
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Igarashi Kiyohiko
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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YOSHIDA MAKOTO
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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KAWAI Rie
Graduate School of Agricultural and Life Sciences, University of Tokyo
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TANI Tomomi
Graduate School of Agricultural and Life Sciences, University of Tokyo
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Kawai Rie
Department Of Biomaterials Sciences Graduate School Of Agricultural And Life Sciences University Of
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Tani Tomomi
Department Of Biomaterials Sciences Graduate School Of Agricultural And Life Sciences University Of
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Samejima Masahiro
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Nagasawa Hiromichi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences Universi
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Nagasawa Hiromichi
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Samejima M
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Yoshida Makoto
Faculty Of The Integated Arts And Sciences Hiroshima University
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Yoshida Makoto
Department Of Environmental And Natural Resource Science Faculty Of Agriculture Tokyo University Of
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Yoshida Makoto
Fac. Of Agriculture Tokyo Univ. Of Agriculture And Technol.
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Igarashi Kiyohiko
Department Of Biomaterial Sciences Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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