A Recombinant Molt-inhibiting Hormone of the Kuruma Prawn Has a Similar Secondary Structure to a Native Hormone : Determination of Disulfide Bond Arrangement and Measurements of Circular Dichroism Spectra(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
In crustaceans, molt-inhibiting hormone (MIH) is presumed to regulate molting through suppressing synthesis and/or secretion of ecdysteroids by the Y-organ. Recently, a recombinant MIH of the kuruma prawn Penaeus japonicus was produced in E. coli. To approximate the secondary structure of native and recombinant MIH of P. japonicus containing six cysteine residues, the arrangements of disulfide bridges in both MIHs were determined by characterizing their enzymatic digests, and their circular dichroism spectra were measured. The arrangements of disulfide bonds in both MIHs were determined to be identical, and they were linked between Cys^7 and Cys^<44>, Cys^<24> and Cys^<40>, and Cys^<27> and Cys^<53>. The circular dichroism spectra of both MIHs were very close, and demonstrated that they were rich in α-helix. α-Helix contents in native and recombinant MIHs were calculated to be 49.3% and 46.0%, respectively. All these results strongly suggested that the recombinant MIH was folded in the same manner as the native MIH.
- 社団法人日本農芸化学会の論文
- 2001-08-23
著者
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Ohira Tsuyoshi
Japan International Research Center for Agricultural Sciences
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Ohira Tsuyoshi
Graduate School Of Marine Science And Technology Tokyo University Of Marine Science And Technology
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Nagasawa Hiromichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Ohira T
Kanagawa Univ. Kanagawa Jpn
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Ohira Tsuyoshi
Laboratory Of Genome Science Graduate School Of Marine Science And Technology Tokyo University Of Ma
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Ohira Tsuyoshi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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KATAYAMA Hidekazu
Institute for Protein Research, Osaka University
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KATAYAMA Hidekazu
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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NAGATA Koji
Biotechnology Research Center, The University of Tokyo
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Katayama H
Univ. Tokyo Tokyo Jpn
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Ohira Tsuyoshi
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nagata Kazuhiro
Depatrment Of Molecular And Cellular Biology Institute For Frontier Medical Sciences Kyoto Universit
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長澤 寛道
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nagata Koji
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences The Univ
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Nagata Koji
Biotechnology Research Center University Of Tokyo
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Nagata Koji
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Nagata Koji
Biotechnology Research Center The University Of Tokyo
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Katayama Hidekazu
Department Of Applied Biochemistry Institute Of Glycoscience Tokai University
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Nagasawa Hiromichi
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Katayama Hidekazu
Department Applied Of Biochemistry Tokai University
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