Identification of Chitin in the Prismatic Layer of the Shell and a Chitin Synthase Gene from the Japanese Pearl Oyster, Pinctada fucata
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概要
- 論文の詳細を見る
The shell of the Japanese pearl oyster, Pinctada fucata, consists of two layers, the prismatic layer on the outside and the nacreous layer on the inside, both of which comprise calcium carbonate and organic matrices. Previous studies indicate that the nacreous organic matrix of the central layer of the framework surrounding the aragonite tablet is β-chitin, but it remains unknown whether organic matrices in the prismatic layer contain chitin or not. In the present study, we identified chitin in the prismatic layer of the Japanese pearl oyster, Pinctada fucata, with a combination of Calcofluor White staining with IR and NMR spectral analyses. Furthermore, we cloned a cDNA encoding chitin synthase (PfCHS1) that produces chitin, contributing to the formation of the framework for calcification in the shell.
- 社団法人 日本農芸化学会の論文
- 2007-07-23
著者
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SUZUKI Michio
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The Universit
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Nagasawa Hiromichi
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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SAKUDA Shohei
Department of Applied Biological Chemistry, University of Tokyo
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Sakuda Shohei
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nagasawa Hiromichi
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Nagasawa Hiromichi
Dep. Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Univ. Of
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Sakuda Shohei
Dep. Of Applied Biological Chemistry The Univ. Of Tokyo
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Suzuki Michio
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Suzuki Michio
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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SAKUDA Shohei
Department of Agricultural Chemistry, The University of Tokyo
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