Kinetic Analysis of a Chitinase from Red Sea Bream, Pagrus major
スポンサーリンク
概要
- 論文の詳細を見る
Kinetic analysis was done on the 46-kDa chitinase (EC 3.2.1.14) purified from the stomach of red sea bream, Pagrus major, using glycolchitin and N-acetylchitooligosaccharides (GlcNAcn, n=2–6) as substrates. High activity was observed at two pHs, such as 2.5 and 9.0, toward glycolchitin as seen in other insect chitinases, and also at both pH 2.5 and 5.0 even toward a short substrate, N-acetylchitopentasaccharide. Allosamidin competitively inhibited chitinase with Ki value of 0.0214 μM at pH 2.5 and 0.0024 μM at pH 9.0 in the reaction of glycolchitin. Substrate inhibition was observed in the reaction of N-acetylchitopentasaccharide. The anomeric forms of the products from N-acetylchitooligosaccharides were analyzed to be β anomer by the high pressure liquid chromatography (HPLC) method. The data for both β-anomer formation and allosamidin inhibition suggest that red sea bream chitinase belongs to family 18 of glycosyl hydrolases. This suggestion is also supported by the results for the N-terminal amino acid sequence.
- 社団法人 日本農芸化学会の論文
- 2004-06-23
著者
-
KONO Michiko
Fisheries Research Laboratory, Graduate School of Agricultural and Life Sciences, The University of
-
Kono Michiko
Fisheries Research Laboratory Graduate School Of Agricultural And Life Sciences The University Of To
-
SAKUDA Shohei
Department of Applied Biological Chemistry, University of Tokyo
-
KARASUDA Shuji
Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi Univ
-
YAMAMOTO Kosuke
Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi Univ
-
KOGA Daizo
Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi Univ
-
Sakuda Shohei
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
-
Kono Michiko
Fisheries Research Labolatory Graduate School Of Agricultural And Life Sciences University Of Tokyo
-
Koga Daizo
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
-
Karasuda Shuji
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
-
Yamamoto Kosuke
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
-
SAKUDA Shohei
Department of Agricultural Chemistry, The University of Tokyo
関連論文
- The Accumulation of Docosahexaenoic Acid in Rotifers by Feeding the DHA-enriched Yeast, Pichia methanolica HA-32
- Well Developed Splenic Ellipsoids in Japanese Conger Conger myriaster
- Production Site of Red Sea Bream Pagrus major Intraperitoneal Free Cells:Morphological Study
- Long-term Cell Culture of Resident Peritoneal Macrophages From Red Sea Bream Pagrus major
- Heterogeneity of Peritoneal Cells in Marine Teleosts
- Resident Peritoneal Cells of Red Sea Bream Pagrus major
- Structures and Biosynthesis of Aflastatins: Novel Inhibitors of Aflatoxin Production by Aspergillus parasiticus
- Absolute Configuration of Allosamizoline, an Aminocyclitol Derivative of the Chitinase Inhibitor Allosamidin(Organic Chemistry)
- PD33 BIOSYNTHESIS OF MACROLIDE ANTIBIOTIC PATULOLIDES BY Penicillium urticae S11R59 : IDENTIFICATION OF THE ORIGIN OF CARBON ATOMS BY ^C NMR SPECTROSCOPY
- PC88 THE STRUCTURES OF INDUCING FACTORS FOR VIRGINIAMYCIN PRODUCTION IN Streptomyces virginiae
- Kinetic Analysis of a Chitinase from Red Sea Bream, Pagrus major
- Isolation of a cDNA Encoding a Chitinase Family Protein from Cuticular Tissues of the Kuruma Prawn Penaeus japonicus
- Nucleotide Sequences of Genes Encoding Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces(Biochemistry & Molecular Biology)
- Biosynthesis of the Trehalase Inhibitor Trehazolin
- Effects of Aflastatin A, an Inhibitor of Aflatoxin Production, on Aflatoxin Biosynthetic Pathway and Glucose Metabolism in Aspergillus parasiticus
- Blasticidin A as an Inhibitor of Aflatoxin Production by Aspergillus parasiticus
- Gordonan, an Acidic Polysaccharide with Cell Aggregation-Inducing Activity in Insect BM-N4 Cells, Produced by Gordonia sp.
- Structure of Demethylallosamidin as an Insect Chitinase Inhibitor(Organic Chemistry)
- Identification of a novel cuticular protein in the kuruma prawn Penaeus japonicus
- Elevation of the activities of β-N-acetylhexosaminidase isozymes by 20-hydroxyecdysone administration in the kuruma prawn
- Purification and Characterization of β-N-Acetylhexosaminidase from the Liver of a Prawn, Penaeus japonicus
- Chitinolytic Enzyme Activities in the Hepatopancresas, Tail Fan and Hemolymph of Kuruma Prawn Penaeus japonicus during the Molt Cycle
- Kinetics of a Chitinase from a Prawn, Penaeus japonicus(Biological Chemistry)
- Purifications and Some Properties of Chitinase from the Liver of a Prawn, Penaeus japonicus(Biological Chemistry)
- Purification and Some Properties of Chitinase from the Stomach of Japanese Eel, Anguilla japonica(Biological Chemistry)
- Structure of a Novel Disulfide of 2-(N-Acetylcysteinyl)amido-2-deoxy-α-D-glucopyran-osyl-myo-inositol Produced by Streptomyces sp.
- Purification of Allosamidin-sensitive and -insensitive Chitinases Produced by Allosamidin-producing Streptomyces
- New Inducing Factors for Virginiamycin Production from Streptomyces antibioticus
- IM-2,a Butyrolactone Autoregulator, Induces Production of Several Nucleoside Antibiotics in Streptomyces sp. FRI-5
- Isolation and Structure of Isobutyrylleucanicidin Produced by Streptomyces halstedii
- The Structure of Leucanicidin, a Novel Insecticidal Macrolide Produced by Streptomyces halstedii
- Isolation of Abikoviromycin and Dihydroabikoviromycin as Inhibitors of Polyketide Synthase Involved in Melanin Biosynthesis by Colletotrichum lagenarium
- Isolation and Characterization of 2-Nitroimidazole Produced by Streptomyces Species as an Inhibitor of Both Carbonic Anhydrase and Shell Formation in the Barnacle Balanus amphitrite
- Molecular Studies of Family 18 Chitinases in Animals : Structural and Functional Diversity
- Morphological Changes in Insect BM-N4 Cells Induced by Nocardamine
- Preparation of a Demethylallosamidin Isomer Having an N,N'-Diacetylchitobiosyl Moiety and Its Potent Inhibition against Yeast Chitinase
- Synthesis of a New Allosamidin Analog, N,N'-Diacetyl-β-Chitobiosyl Allosamizoline, and Its Inhibitory Activity against Some Chitinases
- Preparation of N-Monoalkyl and O-Acyl Derivatives of Allosamidin, and Their Chitinase Inhibitory Activities
- Ioslation and Structure of a New Butyrolactone Autoregulator from Streptomyces sp. FRI-5
- Comparative Biochemistry of Chitinases-Anomeric Form of the Reaction Products
- Specific Inhibition of Bombyx mori Chitinase by Allosamidin(Biological Chemistry)
- Purification and Properties of β-N-Acetyl-D-glucosaminidase from Alimentary Canal of the Silkworm, Bombyx mori(Biological Chemistry)
- Chitinase Inhibitor Allosamidin Is a Signal Molecule for Chitinase Production in Its Producing Streptomyces : II. Mechanism for Regulation of Chitinase Production by Allosamidin through a Two-component Regulatory System
- Chitinase Inhibitor Allosamidin Is a Signal Molecule for Chitinase Production in Its Producing Streptomyces : I. Analysis of the Chitinase Whose Production Is Promoted by Allosamidin and Growth Accelerating Activity of Allosamidin
- Structures of Grixazone A and B, A-Factor-dependent Yellow Pigments Produced under Phosphate Depletion by Streptomyces griseus
- Absolute Configuration of a Polyol Fragment of Blasticidin A, a Specific Inhibitor of Aflatoxin Production
- Isolation and Some Characterization of an Acidic Polysaccharide with Anti-calcification Activity from Coccoliths of a Marine Alga, Pleurochrysis carterae(Biochemistry & Molecular Biology)
- Chemical Synthesis and Biological Activity of The Gelatinase Biosynthesis-Activating Pheromone of Enterococcus faecalis and Its Analogs(Organic Chemistry)
- Analysis of Hydrolytic Activity of a 65-kDa Chitinase from the Silkworm, Bombyx mori(Biochemistry & Molecular Biology)
- Involvement of AfsA in A-factor Biosynthesis as a Key Enzyme
- Effects of Demethylallosamidin, a Potent Yeast Chitinase Inhibitor, on the Cell Division of Yeast(Biological Chemistry)
- Structures of Allisamidins, Novel Insect Chitinase Inhibitors, Produced by Actinomycetes(Organic Chemistry)
- Purification of a Chitooligosaccharidolytic β-N-Acetylglucosaminidase from Bombyx mori Larvae during Metamorphosis and the Nucleotide Sequence of Its cDNA
- Blasticidin A Derivatives with Highly Specific Inhibitory Activity toward Aflatoxin Production in Aspergillus parasiticus
- Kinetic Analysis of Lysozyme-Inhibitor Complex Formation
- On the Activation Process of Pepsinogen
- On the Mechanism of Lysozyme Catalysis II. Hydrolysis of Sugar Oxazoline
- On the Mechanism of Lysozyme Catalysis I. Hydrolysis of 1-Benzoyl 2-Acetamido-2-Deoxy-β-D-Glucopyranoside
- Allosamidin, a chitinase inhibitor produced by Streptomyces, acts as an inducer of chitinase production in its producing strain
- HPLC Analysis of Anomeric Formation and Cleavage Pattern by Chitinolytic Enzyme
- Purification and Some Properties of Exo-type Fucoidanases from Vibrio sp. N-5
- Induction of Fine Bent-grass Callus and Appearance of Chitinase during the Subculture
- Aflastatin A, a Novel Inhibitor of Aflatoxin Production by Aflatoxigenic Fungi
- Chitinase Gene Expression in Response to Environmental Stresses in Arabidopsis thaliana : Chitinase Inhibitor Allosamidin Enhances Stress Tolerance
- Identification of Chitin in the Prismatic Layer of the Shell and a Chitin Synthase Gene from the Japanese Pearl Oyster, Pinctada fucata
- Dillapiol and Apiol as Specific Inhibitors of the Biosynthesis of Aflatoxin G_1 in Aspergillus parasiticus
- Induction Patterns of Chitinases in Yam Callus by Inoculation with Autoclaved Fusarium oxysporum, Ethylene, and Chitin and Chitosan Oligosaccharides
- Efficiency of Chitinolytic Enzymes in the Formation of Tricholoma matsutake Protoplasts(Biological Chemistry)
- Effects of Detergents on Lysis of Micrococcus lysodeikticus by Lysozyme
- Isolation of Tautomycetin as a Regulator of Secondary Metabolite Production for Penicillium urticae
- Structure of Patulodin, a New Azaphilone Epoxide, Produced by Penicillium urticae
- Endocrocin and Its Derivatives from the Japanese Mealybug Planococcus kraunhiae
- Computer Analysis of Lysozyme-catalyzed Reaction with Chitooligosaccharide
- Note on Steady-state Approximation of Enzyme Kinetics
- Simulation of Biochemical System with Computer Graphics
- INTERACTION OF ACYLATED PROTEIN WITH BIOLOGICAL MEMBRANE
- Comparison of Chitinase Isozymes from Yam Tuber : Enzymatic Factor Controlling the Lytic Activity of Chitinases
- Purification and Characterization of A Novel Chitinase Isozyme from Yam Tuber
- Correlation between Membrane Binding and Phosphorylation by Protein Kinase C of Acylated Protein(Biological Chemistry)
- Kinetics of Chitinase from Yam, Dioscorea opposita THUNB(Biological Chemistry)
- Callus Induction from Yam, Dioscorea opposita Thunb., and The Subculture(Biological Chemistry)
- Dillapiol and Apiol as Specific Inhibitors of the Biosynthesis of Aflatoxin G1 in Aspergillus parasiticus
- Simulation for Activation Process of Pepsinogen
- Molecular Cloning of a Genomic DNA Encoding Yam Class IV Chitinase
- Intracellular Localization of a Class IV Chitinase from Yam
- A cryptic antibiotic triggered by monensin
- Astaxanthin dirhamnoside, a new astaxanthin derivative produced by a radio-tolerant bacterium, Sphingomonas astaxanthinifaciens
- Promomycin, a polyether promoting antibiotic production in Streptomyces spp.
- Inhibitory activity of blasticidin A, a strong aflatoxin production inhibitor, on protein synthesis of yeast : selective inhibition of aflatoxin production by protein synthesis inhibitors
- Isolation of Indole-3-Aldehyde As a Growth Inhibitor of Legionella pneumophila from Diaion HP-20 Resins Used to Culture the Bacteria
- β-N-Acetyl-D-glucosaminidases from Integument of the Silkworm, Bombyx mori: Comparative Biochemistry with the Pupal Alimentary Canal Enzyme
- Purification and Some Properties of Chitinases from Yam, Dioscorea opposita THUMB
- A Novel Synthesis of L-Pyroglutamyl-glycyl-L-arginine-p-nitroanilide Hydrochloride
- Isolation of Indole-3-Aldehyde As a Growth Inhibitor of Legionella pneumophila from Diaion HP-20 Resins Used to Culture the Bacteria
- Search for Microbial Insect Growth Regulators; L-Alanosine as an Ecdysis Inhibitor