Kinetics of a Chitinase from a Prawn, Penaeus japonicus(Biological Chemistry)

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概要

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• Kinetic analysis was done on a chitinase (EC 3.2.1.14) purified from the liver of a prawn, Penaeus japonicus, using N-acetylchitooligosaccharides (GlcNAc_n, n=2 to 6), p-nitrophenyl N-acetylchitooligo-saccharides (pNp-GlcNAc_n, n=1 to 5), and colloidal chitin as the substrates. The enzyme hydrolyzed GlcNAc_4 to two molecules of GlcNAc_2, GlcNAc_5 to GlcNAc_2 plus GlcNAc_3, and GlcNAc_6 by two ways to GlcNAc_2 plus GlcNAc_4 (87%), and two molecules of GlcNAc_3 (13%). Neither GlcNAc_2 nor GlcNAc_3 was hydrolyzed. The Km and k_<cat> were 0.249 mM and 3.38 sec^<-1> for GlcNAc_4, 0.018mM and 2.67sec^<-1> for GlcNAc_5, and 0.005mM and 2.72sec^<-1> for GlcNAc_6, respectively. The cleavage patterns of p-nitrophenyl N-acetylchitooligosaccharides were different from those of the corresponding N-acetylchitooligosaccharides. The enzyme hydrolyzed colloidal chitin to produce mainly GlcNAc_2 and a trace of GlcNAc_3. Allosamidin inhibited prawn chitinase in a competitive way with a Ki of 0.1μM and IC_<50> of 0.14μM. These results suggest that prawn chitinase is an endo-type chitinolytic enzyme, but different from insect chitinase or yam chitinase in the substrate specificity and cleavage pattern.

• 社団法人日本農芸化学会の論文
• 1990-10-23

著者

• Matsui Toru

  Institute Of Applied Biochemistry University Of Tsukuba

• KONO Michiko

  Fisheries Research Laboratory, Graduate School of Agricultural and Life Sciences, The University of

• MATSUI Takashi

  Laboratory of Exercise Biochemistry, University of Tsukuba Graduate School of Comprehensive Human Sc

• Kono M

  Fisheries Rsearch Laboratory Faculty Of Agriculture The University Of Tokyo

• Matsui T

  Institute Of Applied Biochemistry University Of Tsukuba

• KOGA Daizo

  Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi Univ

• Kono M

  Univ. Tokyo Shizuoka Jpn

• Kono M

  Fisheries Research Laboratory Faculty Of Agriculture The University Of Tokyo

• Kono Michiko

  Fisheries Research Labolatory Graduate School Of Agricultural And Life Sciences University Of Tokyo

• IDE Akio

  Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University

• MIZUKI Koichi

  Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University

• SHIMIZU Chiaki

  Fisheries Research Laboratory, Faculty of Agriculture, The University of Tokyo

• Matsui T

  Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of

• Ide A

  Kihara Institute For Biological Research Yokohama City University

• Ide Akio

  Laboratory Of Biochemistry Faculty Of Agriculture Yamaguchi University

• Koga D

  Department Of Biological Science Faculty Of Agriculture Yamaguchi University

• Koga Daizo

  Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers

• Mizuki Koichi

  Laboratory Of Biochemistry Faculty Of Agriculture Yamaguchi University

• Shimizu Chiaki

  Fisheries Research Laboratory Faculty Of Agriculture The University Of Tokyo

• Shimizu Chiaki

  Fisheries Laboratory Faculty Of Agriculture University Of Tokyo

• Matsui Takashi

  Laboratory Of Exercise Biochemistry University Of Tsukuba Graduate School Of Comprehensive Human Sci

• Matsui Takashi

  Laboratory Of Exercise Biochemistry And Neuroendocrinology University Of Tsukuba Graduate School Of Comprehensive Human Sciences

• Kono Michiko

  Fisheries Rsearch Laboratory Faculty Of Agriculture The University Of Tokyo

• Matsui Takashi

  Laboratory of Biomolecular Dynamics, Department of Physics, Kitasato University School of Science

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