Purification and Some Properties of Exo-type Fucoidanases from Vibrio sp. N-5
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概要
- 論文の詳細を見る
Three fucoidanases were purified from Vibrio sp. N-5 by ammonium sulfate fractionation and chromatography with DEAE-Toyopearl 650 M, Sephacryl S-300 HR, and chromatofocusing. The purified enzymes gave a single band on disc polyacrylamide gel electrophoresis. The molecular weights of the enzymes, E-1,E-2,and E-3 were 39,500,68,000,and 68,000,respectively, by SDS polyacrylamide gel electrophoresis and 158,000,68,500,and 67,500 by gel filtration. The enzymes hydrolyzed gagome-fucoidan to give small oligosaccharides containing sulfate as main product.
- 社団法人日本農芸化学会の論文
- 1992-11-23
著者
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KOGA Daizo
Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi Univ
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IDE Akio
Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University
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Ide Akio
Laboratory Of Biochemistry Faculty Of Agriculture Yamaguchi University
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Koga Daizo
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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Furukawa Shin-ichi
Laboratory Of Nutrition Chemistry Department Of Home Economics Hijiyama Women's Junior Cellege
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FUJIKAWA Tatsuo
Laboratory of Nutrition Chemistry, Department of Home Economics, Hijiyama Women's Junior Cellege
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Fujikawa Tatsuo
Laboratory Of Nutrition Chemistry Department Of Home Economics Hijiyama Women's Junior Cellege
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