HPLC Analysis of Anomeric Formation and Cleavage Pattern by Chitinolytic Enzyme
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概要
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The reactions of N-acetylchitooligosaccharides with chitinolytic enzyme were analyzed by HPLC using a Tosoh TSK-Gel amide-80 column with 70% acetonitrile as an eluent. We separated α and β anomeric forms of N-acetyl-chitooligosaccharides, and obtain the following advantages of this HPLC method. 1. We can easily identify the reaction mechanism of chitinolytic enzymes by this method, distinguishing the inverting mechanism showing α anomer formation from the retaining mechanism showing β anomer formation. 2. We can also estimate the cleavage patterns of N-acetyl-chitooligosaccharides by chitinolytic enzymes by using natural substrates.
- 社団法人日本農芸化学会の論文
- 1998-08-23
著者
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KOGA Daizo
Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi Univ
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Arakane Y
Yamaguchi Univ. Yamaguchi Jpn
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Koga Daizo
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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Koga Daizo
Laboratory Of Biochemistry Faculty Of Agriculture Yamaguchi University
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YOSHIOKA Takanori
Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University
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ARAKANE Yasuyuki
Research Laboratory, Bankaku So-honpo
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Arakane Yasuyuki
Research Laboratory Bankaku So-honpo Co. Ltd.:laboratory Of Biochemistry Department Of Biological Sc
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Yoshioka Takanori
Laboratory Of Biochemistry Faculty Of Agriculture Yamaguchi University
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