Comparison of Chitinase Isozymes from Yam Tuber : Enzymatic Factor Controlling the Lytic Activity of Chitinases
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概要
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To evaluate the anti-pathogen activity of chitinases, we developed a new method for measuring the lytic activity, and investigated the correlation of the lytic activity with the enzymatic properties by using four chitinase isozymes, Chitinases E, F, H1 and G, which had been purified from yam tubers by column chromatography. Chitinases E, F and H1 had high lytic activity against the plant pathogen, Fusarium oxysporum, but Chitinase G did not. Chitinase E, which is the family 19 chitinase, was similar to Chitinases F and G in its antigenecity, but not to Chitinase H1 or H2. Chitinases H1 and H2 were recognized by the anti-Bombyx mori chitinase antibody, suggesting that Chitinases H1 and H2 are family 18 chitinases like B. mori chitinases. Chitinases E, F and H1 had two optimum pH ranges of 3-4 and 7.5-9 toward glycolchitin, but Chitinase G had only one optimum pH value of 5. Chitinases E, F and H1 had higher affinity to the polymer substrate, glycolchitin, than Chitinase G. These results suggest that the lytic activity of plant chitinases may be related to the chitin affinity and probably to the characteristic optimum pH value, or two values, but not related to its classification. The correlation of the lytic activity of a chitinase isozyme with its elicitor specificity is also discussed.
- 社団法人日本農芸化学会の論文
- 2000-04-23
著者
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SASAKI Yuji
Laboratory of Veterinary Pathology, Faculty of Agriculture, Yamaguchi University
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HOSHIKA Hironori
Laboratory of Biochemistry, Faculty of Agriculture, Yamaguchi University
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Koga Daizo
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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Arakane Yasuyuki
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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KAWASHIMA Natsumi
Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi Univ
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FUJIYA-TSUJIMOTO Chika
Laboratory of Biochemistry, Department of Biological Science, Faculty of Agriculture, Yamaguchi Univ
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Kawashima Natsumi
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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Fujiya-tsujimoto Chika
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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Koga Daizo
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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Hoshika Hironori
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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Sasaki Yuji
Laboratory Of Biochemistry Department Of Biological Science Faculty Of Agriculture Yamaguchi Univers
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Sasaki Yuji
Laboratory Of Applied Microbiology Faculty Of Agriculture Hokkaido University
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