Isolation of a cDNA Encoding a Chitinase Family Protein from Cuticular Tissues of the Kuruma Prawn Penaeus japonicus
スポンサーリンク
概要
- 論文の詳細を見る
To identify and characterize a chitinase related to molting in the Kuruma prawn Penaeus japonicus, we searched for chitinase-encoding cDNA expressed in cuticular tissues. Using two degenerate oligonucleotide primers derived from the two conserved regions of the chitinase protein family, a RT (reverse transcription)-PCR product was obtained. This product was used as a probe to screen a cDNA library from a mixture of the tail fan and blade-two tissues which consist mainly of chitinous exoskeleton and underlying epidermis. A positive cDNA clone was analyzed for the sequence. This clone contains an open reading frame for a protein (named Pjchi-2) of 527 amino acids which exhibits sequence similarity to known chitinases. A typical signals sequence could not be found in the Pjchi-2 sequence. Significant accumulation of Pjchi-2 mRNA was detected in the mixture of the tail fan and blade prior to molting, whereas the transceipt level was much lower during the intermolt stage. This observation suggests tht Pjchi-2 plays a role in molting. The mRNA was not detected in the hepatopancreas. This expression pattern of Pjchi-2 makes a contrast to that of Pjchi-1 which encodes amother chitinase family protein in P. japonicus, and is expressed in the hepatopancreas but not in the tail fan or blade.
- 社団法人日本動物学会の論文
著者
-
KONO Michiko
Fisheries Research Laboratory, Graduate School of Agricultural and Life Sciences, The University of
-
Kono Michiko
Fisheries Research Laboratory Faculty Of Agriculture The University Of Tokyo
-
Watanabe T
Kyoto Univ. Kyoto Jpn
-
Watanabe Toshiki
Laboratory Of Tumor Cell Biology Department Of Medical Genome Sciences Graduate School Of Frontier S
-
Kono Michiko
Fisheries Research Labolatory Graduate School Of Agricultural And Life Sciences University Of Tokyo
-
Watanabe T
Department Of Anatomy Asahikawa Medical College
-
Watanabe Toshiki
Laboratory Of Tumor Cell Biology Department Of Medical Genome Science Graduate School Of Frontier Sc
-
Watanaba Toshiki
Laboratory of Molecular Biology of Marine Organisms, Ocean Research Institute, The University of Tokyo
関連論文
- The Accumulation of Docosahexaenoic Acid in Rotifers by Feeding the DHA-enriched Yeast, Pichia methanolica HA-32
- Well Developed Splenic Ellipsoids in Japanese Conger Conger myriaster
- Production Site of Red Sea Bream Pagrus major Intraperitoneal Free Cells:Morphological Study
- Long-term Cell Culture of Resident Peritoneal Macrophages From Red Sea Bream Pagrus major
- Heterogeneity of Peritoneal Cells in Marine Teleosts
- Resident Peritoneal Cells of Red Sea Bream Pagrus major
- Kinetic Analysis of a Chitinase from Red Sea Bream, Pagrus major
- Catechin, green tea component, causes caspase-independent necrosis-like cell death in chronic myelogenous leukemia
- 41 Pseudohypoparathyroidism type Ib with atypical features : the relationship with DNA methylation pattern in XLαs region of GNAS1 gene
- TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling
- Preventing the unfolded protein response via aberrant activation of 4E-binding protein 1 by versipelostatin
- Serum level of soluble CD30 correlates with the aggressiveness of adult T-cell leukemia/lymphoma
- Impaired Tax-specific T-cell responses with insufficient control of HTLV-1 in a subgroup of individuals at asymptomatic and smoldering stages
- Isolation of a cDNA Encoding a Chitinase Family Protein from Cuticular Tissues of the Kuruma Prawn Penaeus japonicus
- HUNTING FOR VANADIUM-ASSOCIATED PROTEINS FROM ASCIDIAN SERUM(Physiology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- HUNTING FOR VANADIUM-ASSOCIATED PROTEINS FROM ASCIDIAN SERUM(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Molecular Cloning of a Molt-Inhibiting Hormone cDNA from the Kuruma Prawn Penaeus japonicus
- PREPARATION OF MONOCLONAL ANTIBODY AGAINST THE SPERM MOTILITY-INDUCING SUBSTANCE IN EGG-JELLY OF THE NEWT, CYNOPS PYRRHOGASTER(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Identification of a novel cuticular protein in the kuruma prawn Penaeus japonicus
- Elevation of the activities of β-N-acetylhexosaminidase isozymes by 20-hydroxyecdysone administration in the kuruma prawn
- CRUSTOCALCIN : A STUDY ON POTENTIAL FUNCTION OF A SKELETAL CA^-BINDING PROTEIN OF KURUMA PRAWN PENAEUS JAPONICUS(Biochemistry)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Localization of a Ca^ binding protein DD4 in the calcified exoskeleton of kuruma prawn Penaeus japonicus.(Biochemistry)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Ca^ binding capacity of DD4 protein which is expressed during calcification of the exoskeleton of the kuruma-prawn Penaeus japonicus(Biochemistry)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- Purification and Characterization of β-N-Acetylhexosaminidase from the Liver of a Prawn, Penaeus japonicus
- Chitinolytic Enzyme Activities in the Hepatopancresas, Tail Fan and Hemolymph of Kuruma Prawn Penaeus japonicus during the Molt Cycle
- Kinetics of a Chitinase from a Prawn, Penaeus japonicus(Biological Chemistry)
- Purifications and Some Properties of Chitinase from the Liver of a Prawn, Penaeus japonicus(Biological Chemistry)
- Purification and Some Properties of Chitinase from the Stomach of Japanese Eel, Anguilla japonica(Biological Chemistry)
- Molecular Studies of Family 18 Chitinases in Animals : Structural and Functional Diversity
- Expression of Membrane-Bound and Soluble Guanylyl Cyclase mRNAs in Embryonic and Adult Retina of the Medaka Fish Oryzias latipes
- Geographic Color Variation in Two Geotrupes Dung Beetles : A Further Study(General Entomology)
- A Quantitative Analysis of Geographic Color Variation in Two Geotrupes Dung Beetles
- Successful Unrelated Cord Blood Transplantation in an Infant with Wiskott-Aldrich Syndrome following Recurrent Cytomegalovirus Disease
- Characteristics of Sperm Motility Induced on the Egg-Jelly in the Internal Fertilization of the Newt, Cynops pyrrhogaster(Reproductive Biology)
- MOLECULAR ANALYSIS OF GALAXIN, A SOLUBLE SKELETAL MATRIX PROTEIN IN THE SCLERACTINIAN CORAL GALAXEA FASCICULARIS(Biochemistry)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- EXPRESSION OF PARTICULATE AND SOLUBLE GUANYLYL CYCLASE mRNA IN THE MEDAKA RETINA(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- The role of proteinacious factor and potassium ion in the initiation of sperm motility of the newt, Cynops pyrrhogaster.(Developmental Biology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Molecular analysis of a protein in the skeletal organic matrix of the hermatypic coral, Galaxea fascicularis(Biochemistry)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- ANALYSIS OF SKELETAL PROTEINS IN THE HERMATYPIC CORAL Galaxea fascicularis(Biochemistry)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- Substances for the Initiation of Sperm Motility in Egg-Jelly of the Japanese Newt, Cynops pyrrhogaster(Developmental Biology)
- CHROMOSOMAL STREPTOMYCIN ADENYLYLTRANSFERASE AND ITS CODING GENE IN BACILLUS SUBTILIS
- Effect of Granulocyte Colony-Stimulating Factor on Bone Metabolism during Peripheral Blood Stem Cell Mobilization
- Hodgkin's Lymphoma and CD30 Signal Transduction
- A novel domain in the CD30 cytoplasmic tail mediates NFkB activation
- THALLIUM-201 MYOCARDIAL IMAGING FOR EVALUATION OF LEFT VENTRICULAR HYPERTROPHY AND DILATATION: COMPARISON WITH ECHOCARDIOGRAPHY : Nuclear Cardiology : PROCEEDINGS OF THE 44th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- DEVELOPMENT OF PLASMID VECTORS IN BACILLUS SUBTILIS. GENE ORGANIZATION OF DRUG-RESISTANCE PLASMIDS IN B. SUBTILIS
- CHARACTERIZATION OF BACILLUS SUBTILIS CLONING VECTOR pTZ12 ORIGINATED FROM CORYNEBACTERIUM XEROSIS STRAIN
- The side population, as a precursor of Hodgkin and Reed-Sternberg cells and a target for nuclear factor-κB inhibitors in Hodgkin's lymphoma
- Newly Devised Measuring Device for Vertical Maxillomandibular Registration
- 239 CASE REPORT : Vitamin D deficiency in 9-year-old girl
- 171 A family of primary adrenal insuffidency : a case report
- EPIDEMIOLOGICAL STUDY ON THE AGING OF PHYSIQUE AND PHYSICAL FUNCTIONS
- 12.STUDIES ON THE ENERGY COST OF VARIOUS SPORTS (VI) ON THE INTENSITY OF EXERCISE OF THE GYMNASTICS