The Accumulation of Docosahexaenoic Acid in Rotifers by Feeding the DHA-enriched Yeast, Pichia methanolica HA-32
スポンサーリンク
概要
- 論文の詳細を見る
In order to improve the nutritional value of rotifers as the feed for juvenile fish, a yeast, Pichia methanolica HA-32, enriched with docosahexaenoic acid (DHA) was fed to rotifers. The composition of the cultivation medium (pH6) for enrichment of yeast with n-3 highly unsaturated fatty acids were as follows; 5% glucose, 2% yeast extract, 0.5% choline, 1% K2HPO4, 3% free fatty acid rich in DHA. Yeast was incubated at 25°C for 3 d. After incubation, the yeast contained 246 mg of total lipids and 69 mg of DHA per g dry cells. The DHA content in the rotifers cultured with the DHA-enriched yeast reached a maximum after 6 h-feeding. As a result, when 8.0 g (wet basis) of the DHA-enriched yeast (DHA content 170 mg) in 10 L of cultivating water was fed to 800 individuals/mL of rotifers and cultivated for 6 h, 240 mg of total lipids, 51 mg of DHA and 15 mg of EPA in rotifers were recovered. Moreover, the accumulation of DHA in rotifer by feeding the DHA-enriched yeast was more efficient than that by the commercial powdered oil containing the comparable amount of DHA to the DHA-enriched yeast.
- 日本油化学会の論文
- 2004-09-01
著者
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Fujimoto Kenshiro
Graduate School Of Agricultural Science Tohoku University
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Kimura Fumiko
東北大学 農学研究科
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Kimura Fumiko
Grad. Sch. Agr. Sci. Tohoku Univ.
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ENDO Yasushi
Department of Materials Science and Engineering, Graduate School of Engineering, Osaka University
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Endo Y
School Of Bionics Tokyo University Of Technology
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Endo Yasushi
東北大学 大学院農学研究科
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FUJIMOTO Kenshiro
Department of Food and Nutrition, Koriyama Women's University
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AOKI Hideyuki
Research Laboratory, Ikeda Tohka Industries Co., Ltd
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FURUYA Yuji
Research Laboratory, Ikeda Food Research Co. Ltd.
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KONO Michiko
Fisheries Research Laboratory, Graduate School of Agricultural and Life Sciences, The University of
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FURUKAWA Kiyoshi
Department of Aquatic Biosciences, Graduate School of Agricultural and Life Sciences, The University
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Yasushi Endo
Grakuate School Of Agricultural Science Tohoku University
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Yasushi Endo
Laboratory Of Food And Bio-molecular Science Graduate School Of Agricultural Science Tohoku Universi
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Endo Y
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Endo Yasushi
Faculty Of Agriculture Tohoku University
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Kimura F
Graduate School Of Agricultural Science Tohoku University
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Fujimoto K
Graduate School Of Agricultural Science Tohoku University
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Furuya Yuji
Research Laboratory Ikeda Food Research Co. Ltd.
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Furuya Yuji
Research Laboratory Ikeda Toka Kogyo Co.
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Furuya Y
Department Of Biotechnology Faculty Of Engineering Tottori University:research Laboratory Ikeda Food
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Aoki H
Iwate Univ. Iwate Jpn
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Aoki Hideyuki
Research Laboratory Ikeda Food Research Co. Ltd.
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Aoki H
Ikeda Food Res. Co. Ltd. Hiroshima Jpn
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Fujimoto Kenshiro
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Kono Michiko
Fisheries Research Labolatory Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Fujimoto Kenshiro
Faculty Of Agriculture Tohoku University
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Kimura F
Laboratory Of Food And Biomolecular Science Graduate School Of Agricultural Science Tohoku Universit
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Aoki Hideyuki
Research Institute For Food Science Kyoto University
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Kimura Fumiko
Laboratory Of Food And Bio-molecular Science Graduate School Of Agricultural Science Tohoku Universi
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Endo Yasushi
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Furukawa Kiyoshi
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Furukawa Kiyoshi
Department Of Aquatic Biosciences Graduate School Of Agricultural And Life Sciences The University O
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Kono Michiko
Fisheries Research Laboratory Graduate School Of Agricultural And Life Sciences The University Of To
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FUJIMOTO Kenshiro
Department of Applied Biochemistry, Faculty of Agriculture, Tohoku University
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