Detection of Picomole Levels in Lipid Hydroperoxides by a Chemiluminescence Assay
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概要
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A highly sensitive and simple chemiluminescent method for the quantitation of lipid hydroperoxides at the picomole level is described. The method is based on detecting the Chemiluminescence generated during the oxidation of luminol by the reaction with hydroperoxide and cytochrome c under mild conditions. A semilogarithmic relationship was observed between the hydroperoxide added and the Chemiluminescence produced. For lipid hydroperoxides, cytochrome c was a most favorable catalyst for generating the Chemiluminescence, rather than cytochrome c heme peptide and horseradish peroxidase. This method had high sensitivity to methyl linoleate hydroperoxide, arachidonic acid hydroperoxide and cholesterol hydroperoxide, but low to t-butyl hydroperoxide, t-butyl perbenzoate, diacyl peroxides (lauroyl peroxode and benzoyl peroxide) and dialkyl peroxides (di-t-butyl peroxide and dicumyl peroxide).
- 社団法人 日本農芸化学会の論文
著者
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FUJIMOTO Kenshiro
Department of Food and Nutrition, Koriyama Women's University
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Fujimoto K
Department Of Applied Biological Chemistry Tohoku University
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Miyazawa Teruo
Department Of Food Chemistry Faculty Of Agriculture Tohoku University
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Miyazawa Teruo
Department Of Applied Biochemistry Tohoku University School Of Agriculture
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KANEDA Takashi
Laboratory of Food and Nutrition, Kohriyama Womens College
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FUJIMOTO Kenshiro
Department of Applied Biochemistry, Faculty of Agriculture, Tohoku University
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FUJIMOTO Kenshiro
Department oj Food Chemistry, Tohoku University
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