Method for Quantitative Determination of Cerebroside in "Plant Ceramide" Foodstuffs by High Performance Liquid Chromatography with Evaporative Light Scattering Detection
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概要
- 論文の詳細を見る
Normal-phase high performance liquid chromatography was developed with evaporative light scattering detection (HPLC-ELSD) for determining cerebroside (monoglycosylceramide) present in commercially available “Plant ceramide”. Authentic cerebroside, isolated from wheat flour by preparative HPLC, served as the standard. A standard curve was obtained for what at 1 to 40 μg of cerebroside, the equation for which was y=axb (x: cerebroside weight; y: peak area). The regression correlation coefficients was above 0.997. Prior to making determination, food samples were saponified so as to remove glycerolipids, and thus improve the precision of cerebroside. Determination on three foodstuffs each containing cerebroside, the present method was conducted and evaluated has on the resulted. Statistically significant intra- and inter-laboratory variation in the data were analyzed. The present HPLC-ELSD was shown quite effective for accurately determining cerebroside content in “Plant ceramide” food samples.
- 日本油化学会の論文
- 2002-05-01
著者
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MIYAZAWA Teruo
Graduate School of Agricultural Science, Tohoku University
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Miyazawa T
Tohoku Univ. Miyagi Jpn
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Miyazawa T
Tohoku Univ. Sendai Jpn
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Miyazawa Teruo
Department Of Food Chemistry Faculty Of Agriculture Tohoku University
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KASHIMA Minoru
NOF Corporation
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Nakagawa Kiyotaka
Graduate School Of Agriculture Tohoku Univ.
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KASHIMA Minoru
Food Research Laboratory, NOF CORPORATION
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SUGAWARA Tatsuya
Graduate School of Agriculture, Tohoku Univ.
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MURAKAMI Chiaki
JAPAN INSTITUTE OF OILS & FATS, OTHER FOODS INSPECTION (FOUNDATION)
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MIYASHITA Rumiko
CENTRAL LABORATORY NIPPON FLOUR MILLS CO., LTD.
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ONO Jisaburo
CENTRAL LABORATORY NIPPON FLOUR MILLS CO., LTD.
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DESCHAMPS F.
Laboratoires LAVIPHARM
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CHAMINADE P.
Laboratoires de Chimie Analytique, Faculte de Pharmacie, Universite Paris Sud
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Chaminade P.
Laboratoires De Chimie Analytique Faculte De Pharmacie Universite Paris Sud
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Nakagawa K
Biodynamic Chemistry Graduate School Of Life Sci. And Agriculture Tohoku Univ.
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Miyazawa Teruo
Tohoku Univ.
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Murakami Chiaki
Japan Institute Of Oils & Fats Other Foods Inspection (foundation)
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Kinoshita Mikio
Dep. Of Agricultural And Life Sci. Obihiro Univ. Of Agriculture And Veterinary Medicine
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Sugawara Tatsuya
Div. Of Applied Biosciences Graduated School Of Agriculture Kyoto Univ.
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Miyashita Rumiko
Central Laboratory Nippon Flour Mills Co. Ltd.
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ONO Jisaburo
Nippon Flour Mills Co., Ltd., Central Laboratory
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Miyazawa Teruo
Graduate School Of Agricultural Sci. Tohoku Univ.
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Ono Jisaburo
Nippon Flour Mills Co. Ltd. Central Laboratory
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Sugawara Tatsuya
Graduate School Of Agriculture Kyoto Univ.
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Murakami Chiaki
Japan Inst. Of Oil & Fats Other Foods Inspection Foundation
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Masuko Tsuyoshi
Department Of Surgery Division Gi And Colorectal Surgery Research Unit Of Biological Regulation And
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Masuko Tsuyoshi
Department Of Surgery Tohoku University Graduate School Of Medicine
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NAKAGAWA Kiyotaka
Graduate School of Agricultural Science, Tohoku University
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