Expression of 135-kDa Insecticidal Protein Gene from Bacillus thuringiensis in the Yeast Saccharomyces cerevisiae

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概要

• 論文の詳細を見る

• Bacillus thuringiensis subsp. aizawai produces 130-kDa and 135-kDa (CryIA(a)) insecticidal proteins. When Saccharomyces cerevisiae was transformed by the vector carrying a cryIA(a) gene, the gene expression could not be observed. When the 5'-upstream region from the initiation codon was removed using a synthetic oligonucleotide, the CryIA(a) protein was successfully synthesized in yeast. The yeast extract containing CryIA(d) protein had insecticidal activity against Plutella xylostella larvae.

• 社団法人日本農芸化学会の論文
• 1996-09-23

著者

• TOYODA Masatake

  National Institute of Health Sciences

• Saito Yukio

  National Institute Of Health Sciences

• TANIMURA Akio

  Showa Women's University

• Yoshida Yuzo

  School Of Pharmaceutical Sciences Mukogawa Women's University

• Yoshida Y

  Biotechnology Laboratory Sumitomo Chemical Co. Ltd.

• Yoshida Y

  Tokyo Metropolitan Inst. Of Medical Sci. Tokyo

• Tanimura Akio

  Showa Woman's University

• Yabusaki Y

  Sumitomo Chemical Co. Ltd. Hyogo Jpn

• Yabusaki Yoshiyasu

  Biotechnology Laboratory Sumitomo Chemical Co. Ltd.

• NAKAMURA Keiko

  Biotechnology Laboratory Takarazuka Research Center, Sumitomo Chemical Co., Ltd.

• Nakagawa K

  Biodynamic Chemistry Graduate School Of Life Sci. And Agriculture Tohoku Univ.

• HASHIMOTO HIDEAKI

  Institute for Production Research and Development, Nikka Distilling Co.,

• WATANABE Kayo

  Showa Woman's University

• NAKAGAWA Kei-ichi

  Institute for Production, Research and Development, The Nikka Whisky Co., Ltd.

• Watanabe Kayo

  Showa Woman's University

• Hashimoto H

  Institute For Production Research And Development The Nikka Whisky Co. Ltd.

• Hashimoto Hideaki

  Institute For Production Research And Development Nikka Distilling Co.

• Nakamura Keiko

  Biotechnology Laboratory Takarazuka Research Center Sumitomo Chemical Co. Ltd

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