Capability for Identification of Gamma-Irradiated Bovine Liver by New High Sensitivity Comet Assay
スポンサーリンク
概要
- 論文の詳細を見る
DNA in food will sustain damage by gamma radiation. The detection capability of the high sensitivity comet assay was studied using fluorescence-microscopy. Beef liver was irradiated at a range of 1 Gy to 8 kGy. Single cells were obtained from the irradiated liver, then analyzed by agaros-gel electrophoresis. The pH of the buffer for electrophoresis was pH 13,which is generally utilized for sensitive detection of DNA damage. The pattern formed by DNA was visualized by staining with ethidium bromide. The resulting comets were evaluated with a scale we developed, and Influence Scores were calculated based on the Tice method. It is possible to detect irradiation damage to beef liver at 10 Gy. Together with Influence Score, histogram of comet type is used for detection of irradiation. We elucidated those histograms were useful for distinguishing damage caused by irradiation from that of others.DNA damage can be caused not only by irradiation, but also by the other treatments. Therefore, the respective influences of freezing, preservation, irradiating temperature, atmosphere of irradiation, cooking, and homogenizing devices were also examined.This new comet assay will be a useful method of detecting DNA damage to identify irradiated foods.
- 公益社団法人日本薬学会の論文
- 2000-12-01
著者
-
TOYODA Masatake
National Institute of Health Sciences
-
Saito Akiko
School Of Allid Health Sciences Kitasato University
-
Toyoda M
Jissen Women's Univ.
-
Saito Akiko
Japan Science And Technology Corporation Domestic Research Fellow
-
Toyoda Masatake
Faculty Of Human Life Sciences Jissen Women's University
-
Toyoda M
National Institute Of Health Sciences
-
Miyahara M
Faculty Of Environmental And Symbiotic Sciences Prefectural University Of Kumamoto
-
Miyahara Makoto
National Institute of Health Sciences
-
Ito Hitoshi
The Japan Atomic Energy Research Institute Takasaki Radiation Establishment
-
Takeda Mitsuharu
College Of Bioresource Sciences Nihon University
-
Ito Hitoshi
Japan Atomic Energy Research Institute Takasaki Establishment
-
Toyoda M
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University
-
Miyahara M
Division Of Microbiology National Institute Of Health Sciences
関連論文
- Multiplex PCR法を用いた組換えトウモロコシ5系統からの組換え遺伝子の検知法
- Antitumor Protein-containing Polysaccharides from a Chinese Mushroom Fengweigu or Houbitake, Pleurotus sajor-caju (Fr.) Sings
- Antitumor Activity of Some Polysaccharides Isolated from a Chinese Mushroom, "Huangmo", the Fruiting Body of Hohenbuehelia serotina(Biological Chemistry)
- Activation of the Aryl Hydrocarbon Receptor by Some Vegetable Constituents Determined Using in Vitro Reporter Gene Assay
- Preliminary Screening of the lnhibitory Effect of Food Extracts on Activation of the Aryl Hydrocarbon Receptor Induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin
- Frequency of Mutations of the Transgene, which might Result in the Loss of the Glyphosate-Tolerant Phenotype, was Lowered in Roundup Ready^[○!R] Soybeans
- Constituents in Watercress : Inhibitors of Histamine Release from RBL-2H3 Cells Induced by Antigen Stimulation
- The Effect of Feeding Carrots on Immunoglobulin E Production and Anaphylactic Response in Mice
- 食品中のダイオキシン類測定の前処理における硫酸処理法と多層シリカゲルカラムクロマトグラフ法の比較
- プレパック多層シリカゲルカラムを用いた前処理による食品中のダイオキシン類分析
- Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)(Pharmacognosy)
- A Multi-Prefectural Outbreak of Shigella sonnei Infections Associated with Eating Oysters in Japan
- Gentic Analysis of Nothoapiol Formation in Perilla frutescens
- Chemical Composition of the Essential oil of Perilla frutescens
- Detection of Hydrocarbons in Irradiated Foods
- 市販食用青色1号に含まれる付随色素の解明
- Antitumor Activity and Some Properties of Water-insoluble Hetero-glycans from "Himematsutake," the Fruiting Body of Agaricus blazei Murill(Biological Chemistry)
- Antitumor Activity and Some Properties of Water-soluble Polysaccharides from "Himematsutake," the Fruiting Body of Agaricus blazei Murill(Biological Chemistry)
- 調理による小松菜中のポリ塩化ダイオキシン及びその関連化合物の消長
- 日本における1977年から1998年までの食品由来PCDDs,PCDFs及びCo-PCBsの1日摂取量の減少
- Antiallergic Effect of Apple Polyphenols on the Allergic Model Mouse
- Anti-allergic effect of apple polyphenol on patients with atopic dermatitis : A pilot study
- 市販食用緑色3号中の付随色素の構造解析
- Relationship Between Cardiac Glycoside Contents and Color of Corchorus olitorius Seeds
- ESR Method for the Detection of Irradiated Unboned Meats and Seafood
- Antitumor Active Protein-containing Glycans from the Chinese Mushroom Songshan Lingzhi, Ganoderma tsugae Mycelium
- Antitumor Active Polysaccharides from the Chinese Mushroom Songshan Lingzhi, the Fruiting Body of Ganoderma tsugae
- 遺伝子組換えとうもろこしCBH351のBNラット並びにB10Aマウスへの90日間混餌投与による免疫系への影響
- Gas Chromatographic Determination of Dibutyl-hydroxytoluene in Butter
- Synthetic Studies of Proanthocyanidins. Highly Stereoselective Synthesis of the Catechin Dimer, Procyanidin-B3(Organic Chemistry)
- LC/MSによる5-Enolpyruvylshikimate-3-phosphate Synthaseの酵素活性の測定
- Inhibitory Effects of Apple Polyphenol on Induced Histamine Release from RBL-2H3 Cells and Rat Mast Cells
- Antitumor Activity and Chemical Modification of Polysaccharides from Niohshimeji Mushroom, Tricholma giganteum
- Antitumor-active Heteroglycans from Niohshimeji Mushroom, Tricholoma giganteum
- Expression of 135-kDa Insecticidal Protein Gene from Bacillus thuringiensis in the Yeast Saccharomyces cerevisiae
- 畜産食品中のHPLC-UVによるチアベンダゾール及び5-ヒドロキシチアベンダゾールの定量
- Hydrocarbon Productions in Hexane Solutions of Fatty Acid Methyl Esters Irradiated with Gamma Rays
- 遺伝子組換え食品導入蛋白質(CP4-EPSPS, Cry1Ab)の熱感受性並びにin vitro 分解性試験
- Identification of Irradiation of Boned Chicken by Determination of o-Tyrosine and Electron Spin Resonance Spectrometry
- Evaluation of Several Dosimeters for Identification of Irradiated Foods Using a 5 MeV Electron Beam
- Capability for Identification of Gamma-Irradiated Bovine Liver by New High Sensitivity Comet Assay
- Detection of Irradiation of Meats by HPLC Determination for o-Tyrosine Using Novel LASER Fluorometric Detection with Automatic Pre-column Reaction
- 遺伝子組換え, 非組換え大豆のマウス, ラットへの混餌投与による免疫系への影響
- Determination of o-Tyrosine Production in Aqueous Solutions of Phenylalanine Irradiated with Gamma Ray, Using High Performance Liquid Chromatography with Automated Pre-column Derivatization and LASER Fluorometric Detection
- トウモロコシからの組換え遺伝子の検知法
- Formation of a 3-(Phenylamino)alanine Contaminant in EMS-associated L-Tryptophan
- Antitumor Polysaccharides from a Chinese Mushroom, "Yuhuangmo," the Fruiting Body of Pleurotus citrinopileatus
- QUANTITATIVE EVALUATION OF PASSIVE CUTANEOUS ANAPHYLAXIS (PCA) USING A HAND-HELD SPECTROPHOTOMETER
- Antitumor Active Fucoidan from the Brown Seaweed, Umitoranoo (Sargassum thunbergii)
- Fractionation and Antitumor Activity of Polysaccharides from Grifola frondosa Mycelium
- Antitumor Activity of Some Polysaccharides Isolated from an Edible Mushroom, Ningyotake, the Fruiting Body and the Cultured Mycelium of Polyporous confluens
- Production of New Restriction Endonuclease VpaK11BI Recognizing Sequence 5'-GGWCC-3' in a Haemolysin-less Mutant of Vibrio parahaemolyticus
- Specific Detection of Buckwheat Residues in Processed Foods by Polymerase Chain Reaction
- Specific Detection of Wheat Residues in Processed Foods by Polymerase Chain Reaction
- Collaborative Evaluation of Two Methods for the Detection of Shigella sonnei in Oysters and Young Corn
- コ-ヒ-豆及び穀類中のオクラトキシンAの迅速分析法
- Isolation and Characterization of Restriction Endonuclease in Plesiomonas shigelloides and Aermonas Species
- Simple HPLC Determination of Aflatoxins B_1, B_2, G_1 and G_2 in Nuts and Corn
- 4-(N,N-dimethylaminosulhonyl)-7-fluoro-2,1,3-benzoxadiazole(DBD-F)を用いたプレカラムHPLC法によるとうもろこし中のフモニシンB1,B2の定量〔英文〕
- Essential Oil Composition of Hybrids and Amphidiploids of Japanese Wild Perilla
- Lipase-Catalyzed Kinetic Resolution of (±)-cis-Flavan-4-ol and Its Acetate : Synthesis of Chiral 3-Hydroxyflavanones(Organic Chemistry)