Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)(Pharmacognosy)
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概要
- 論文の詳細を見る
Ethanol extracts of lyophilized vegetables were tested for inhibition of human lanosterol synthase (hOSC) in order to find the compounds to suppress cholesterol biosynthesis. Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highest inhibition (55% inhibition at 300μg/ml). Examination of activity variation among eight taro cultivars indicated that "Aichi-wase" and "Yatsugashira" had the most potent activity for hOSC inhibition. In order to identify the active constituent of taro, ethanol extracts of "Aichi-wase" were partitioned with hexane and aqueous methanol, and fractionated by silica gel column chromatography. Inhibitory activity was concentrated in two major active fractions. Further purification of these fractions by preparative HPLC gave three monogalactosyldiacylglycerols and five digalactosyldiacylglycerols as active compounds that showed 28 to 67% inhibitory activities at the concentration 300μg/ml.
- 公益社団法人日本薬学会の論文
- 2005-02-01
著者
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合田 幸弘
国立医薬品食品衛生研究所
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GODA Yukihiro
National Institute of Health Sciences
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TOYODA Masatake
National Institute of Health Sciences
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Shibuya Masaaki
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Ebizuka Yutaka
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Sato Y
Division Of Food National Institute Of Health Sciences
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Goda Y
National Institute Of Health Sciences
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INAKUMA Takahiro
Research Institute, Kagome Co., Ltd.,
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SUGANUMA Hiroyuki
Research Institute, Kagome Co., LTD.
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Toyoda M
Jissen Women's Univ.
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Toyoda Masatake
Faculty Of Human Life Sciences Jissen Women's University
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Sakano Yuichi
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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TANAKA Rie
National Institute of Health Sciences
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MATSUGA Motoh
National Institute of Health Sciences
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Goda Yukihiro
National Inst. Of Health Sciences
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Toyoda M
National Institute Of Health Sciences
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Shinose Mayumi
Research Center For Biological Function The Kitasato Institute And Graduate School Of Pharmaceutical
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Ebizuka Y
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Ebizuka Yutaka
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Shibuya Masaaki
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Inakuma T
Kagome Co. Ltd.
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Inakuma Takahiro
Res. Inst. Kagome Co. Ltd.
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Suganuma H
Research Institute Kagome Co. Ltd.
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Suganuma Hiroyuki
Research Institute Kagome Co. Ltd.
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Takeda Mitsuharu
College Of Bioresource Sciences Nihon University
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Inakuma Takahiro
Research Institute Kagome Co. Ltd
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Shibata M
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Toyoda M
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University
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Mutsuga Motoh
National Institute of Health Sciences
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Shibuya Masaaki
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Sato Yuji
Graduate School Of Medicine And Pharmaceutical Sciences University Of Toyama
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Sugoh-hagino Masako
Research Center For Biological Function The Kitasato Institute
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Inakuma Takahiro
Research Institute, Kagome Company Ltd.
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Sakano Yuichi
Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
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