Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)(Pharmacognosy)

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概要

• 論文の詳細を見る

• Ethanol extracts of lyophilized vegetables were tested for inhibition of human lanosterol synthase (hOSC) in order to find the compounds to suppress cholesterol biosynthesis. Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highest inhibition (55% inhibition at 300μg/ml). Examination of activity variation among eight taro cultivars indicated that "Aichi-wase" and "Yatsugashira" had the most potent activity for hOSC inhibition. In order to identify the active constituent of taro, ethanol extracts of "Aichi-wase" were partitioned with hexane and aqueous methanol, and fractionated by silica gel column chromatography. Inhibitory activity was concentrated in two major active fractions. Further purification of these fractions by preparative HPLC gave three monogalactosyldiacylglycerols and five digalactosyldiacylglycerols as active compounds that showed 28 to 67% inhibitory activities at the concentration 300μg/ml.

• 公益社団法人日本薬学会の論文
• 2005-02-01

著者

• 合田 幸弘

  国立医薬品食品衛生研究所

• GODA Yukihiro

  National Institute of Health Sciences

• TOYODA Masatake

  National Institute of Health Sciences

• Shibuya Masaaki

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• Ebizuka Yutaka

  Graduate School of Pharmaceutical Sciences, The University of Tokyo

• Sato Y

  Division Of Food National Institute Of Health Sciences

• Goda Y

  National Institute Of Health Sciences

• INAKUMA Takahiro

  Research Institute, Kagome Co., Ltd.,

• SUGANUMA Hiroyuki

  Research Institute, Kagome Co., LTD.

• Toyoda M

  Jissen Women's Univ.

• Toyoda Masatake

  Faculty Of Human Life Sciences Jissen Women's University

• Sakano Yuichi

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• TANAKA Rie

  National Institute of Health Sciences

• MATSUGA Motoh

  National Institute of Health Sciences

• Goda Yukihiro

  National Inst. Of Health Sciences

• Toyoda M

  National Institute Of Health Sciences

• Shinose Mayumi

  Research Center For Biological Function The Kitasato Institute And Graduate School Of Pharmaceutical

• Ebizuka Y

  Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo

• Ebizuka Yutaka

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Shibuya Masaaki

  Graduate School Of Pharmaceutical Sciences The University Of Tokyo

• Inakuma T

  Kagome Co. Ltd.

• Inakuma Takahiro

  Res. Inst. Kagome Co. Ltd.

• Suganuma H

  Research Institute Kagome Co. Ltd.

• Suganuma Hiroyuki

  Research Institute Kagome Co. Ltd.

• Takeda Mitsuharu

  College Of Bioresource Sciences Nihon University

• Inakuma Takahiro

  Research Institute Kagome Co. Ltd

• Shibata M

  Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo

• Toyoda M

  Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University

• Mutsuga Motoh

  National Institute of Health Sciences

• Shibuya Masaaki

  Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo

• Sato Yuji

  Graduate School Of Medicine And Pharmaceutical Sciences University Of Toyama

• Sugoh-hagino Masako

  Research Center For Biological Function The Kitasato Institute

• Inakuma Takahiro

  Research Institute, Kagome Company Ltd.

• Sakano Yuichi

  Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama

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