Molecular Cloning and Characterization of a cDNA for Glycyrrhiza glabra Cycloartenol Synthase
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概要
- 論文の詳細を見る
A cDNA clone (GgCAS1) encoding cycloartenol synthase (CAS) has been isolated from Glycyrrhiza glabra(licorice) by cross-hybridization with that of Pisum sativum CAS as a probe. The deduced amino acid sequence of GgCAS1 exhibits 89%, 83% and 81% identity to those of Pisum sativum, Panax ginseng and Arabidopsis thaliana CASs, respectively. CAS activity has been detected in the homogenate of the yeast transformed with the expression vector containing the open reading frame of GgCAS1. Southerbn blot analysis suggested that at least two CAS genes exist in the licorice genome. In Northern blot analysis, the strong signal for CAS mRNA is detected in the cultured licorice cells of all growth phases, but no significant increase of GAS mRNA expression was observed in the cells treated with the 3-hydroxy-3-methylglutaryl-CoA reductase inhibitor, pravastatin.
- 公益社団法人日本薬学会の論文
- 2000-02-01
著者
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Hayashi H
Univ. Tokyo Tokyo Jpn
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Kushiro Tetsuo
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Shibuya Masaaki
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Ebizuka Yutaka
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Hayashi H
Drug Research Division Dainippon Sumitomo Pharma Co. Ltd.
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Hayashi Hideya
Pharmaceuticals And Biotechnology Laboratory Japan Energy Corporation
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Hayashi H
Pharmaceuticals & Biotechnology Laboratory Japan Energy Corporation
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HIRAOKA Noboru
Niigata College of Pharmacy
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IKESHIRO Yasumasa
Niigata College of Pharmacy
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Hiraoka N
Faculty Of Pharmacy Niigata University Of Pharmacy And Applied Life Sciences
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Hiraoka Noboru
Niigata University Of Pharmacy And Applied Life Sciences
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Ebizuka Y
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Ebizuka Yutaka
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Shibuya Masaaki
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Kushiro Tetsuo
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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渋谷 雅明
Riken Plant Science Center
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HAYASHI Hiroaki
Niigata College of Pharmacy
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MORITA Masayo
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Ikeshiro Yasumasa
Niigata University Of Pharmacy And Applied Life Sciences
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Shibuya Masaaki
Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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