Molecular Cloning and Characterization of Two cDNAs for Glycyrrhiza glabra Squalene Synthase
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概要
- 論文の詳細を見る
Two cDNAs (GgSQS1 and GgSQS2) encoding squalene synthase have been isolated from licorice, Glycyrrhiza glabra L., and characterized. The deduced amino acid sequence of GgSQS1 was 88%, 81%, 78%, 45-44%, and 45-41% identical to those of GgSQS2,Nicotiana, Arabidopsis, mammal and yeast squalene synthases, respectively. Squalene synthase activity was found in the cell-free extracts of Escherichia coli transformed with the recombinant plasmids for GgSQS1 and GgSQS2,respectively. Genomic Southern blot hybridization indicated that there are three squalene synthase genes in the licorice genome. Northern blot analysis showed that GgSQS2 mRNA is mainly expressed during the exponential growth phase of the cultured licorice cells.
- 公益社団法人日本薬学会の論文
- 1999-09-15
著者
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Hayashi H
Univ. Tokyo Tokyo Jpn
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Hayashi H
Drug Research Division Dainippon Sumitomo Pharma Co. Ltd.
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Hayashi Hideya
Pharmaceuticals And Biotechnology Laboratory Japan Energy Corporation
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Hayashi H
Pharmaceuticals & Biotechnology Laboratory Japan Energy Corporation
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HIRAOKA Noboru
Niigata College of Pharmacy
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IKESHIRO Yasumasa
Niigata College of Pharmacy
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Hiraoka N
Faculty Of Pharmacy Niigata University Of Pharmacy And Applied Life Sciences
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Hiraoka Noboru
Niigata University Of Pharmacy And Applied Life Sciences
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HAYASHI Hiroaki
Niigata College of Pharmacy
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HIROTA Akiko
Niigata College of Pharmacy
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Ikeshiro Yasumasa
Niigata University Of Pharmacy And Applied Life Sciences
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