Restriction Fragment Length Polymorphisms of rDNA and Variation of Essential Oil Composition in Atractylodes Plants
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概要
- 論文の詳細を見る
Total DNA was extracted from the leaves of Atractylodes lancea DE CANDOLLE, A. ovata DE CANDOLLE and A. japonica KOIDZUMI ex KITAMURA of various origins and hybridized with digoxigenin-labeled rice ribosomal DNA after digestion with eight different restriction endonucleases. The resulting restriction fragment length polymorphism (RFLP) profiles allowed us to distinguish the three Atractylodes species when DNA was digested with SacI. Although atractylon was detected in the rhizomes of some of the cultivated strains of A. lancea, their RFLP profiles clearly indicate that these plants are not hybrids of A. ovata or A. kaponica.RFLP analysis also revealed the presence of intraspecific variation in DNA sequence of rRNA locus among A. lancea as well as A. japonica.
- 公益社団法人日本薬学会の論文
- 1996-04-15
著者
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Shimizu Ryosuke
Faculty Of Pharmaceutical Sciences Nagoya City University
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KOHDA Hiroshi
The Experimental Station of Medicinal Plants, School of Pharmaceutical Sciences, Hiroshima Universit
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MIZUKAMI Hajime
Faculty of Pharmaceutical Sciences, Nagoya City University
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KOHJYOUMA Mareshige
The Experimental Station of Medicinal Plants, Hiroshima University School of Medicine
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KAWANISHI Fumiaki
Fukuchiyama Experimental Farm, Takeda Chemical Industries, Ltd.,
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HIRAOKA Noboru
Niigata College of Pharmacy
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KAWANISHI Fumiaki
Kyoto Herbal Garden, Takeda Chemical Industries, Ltd.,
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Shimizu R
(pressent Address)biomedical Research Institue Hab Discussion Group
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Kohda H
The Experimental Station Of Medicinal Plant Hiroshima University School Of Medicine
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Hiraoka N
Faculty Of Pharmacy Niigata University Of Pharmacy And Applied Life Sciences
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KOHJYOUMA Mareshige
Izu Experimental Station for Medicinal Plants, National Institute of Health Sciences
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Mizukami Hajime
Faculty Of Pharmaceutical Sciences Nagoya City University
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Mizukami H
Dep. Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City Univ.
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Kohjyouma M
National Inst. Health Sci. Shizuoka Jpn
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Kohjyouma Mareshige
Izu Experimental Station For Medicinal Plants National Institute Of Health Sciences
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Mizukami Hajime
Faculty Of Pharmaceutical Science Nagoya City University
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Kawanishi Fumiaki
Fukuchiyama Experimental Farm Takeda Chemical Industries Ltd.
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Kohda Hiroshi
The Experimental Station Of Medicinal Plans Hiroshima University
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Kohda H
Experimental Station Of Medicinal Plants Graduate School Of Biomedical Sciences Hiroshima University
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