CLONING AND BACTERIAL EXPRESSION OF A GENE ENCODING RIBOSOME-INACTIVATING PROTEINS, KARASURIN-A AND KARASURIN-C, FROM TRICHOSANTHES KIRILOWII VAR. JAPONICA
スポンサーリンク
概要
- 論文の詳細を見る
A genomic DNA clone of karasurin was isolated using the polymerase chain reaction from Trichosanthes kirilowii var. japonica (Cucurbitaceae). The amino acid sequence deduced from the nucleotide sequence was consistent with previously reported sequences of karasurin-A and karasurin-C except for a putative signal peptide and extra amino acids at the C-terminus, neither of which is present in the natural protein. Recombinant karasurin was synthesized in Escherichia coli, in which the cloned karasurin gene was expressed under the control of the trc promotor.
- 公益社団法人日本薬学会の論文
- 1997-06-15
著者
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近藤 俊哉
Faculty Of Pharmaceutical Sciences Nagoya City University
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Kondo T
Research Division The Green Cross Corporation
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Ogihara Yukio
Laboratory Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City University
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Ogihara Yukio
Faculty Of Pharmaceutical Sciences Nagoya City University
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Ogihara Yukio
Graduate School Of Pharmaceutical Sciences Nagoya City University
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IIDA Katsuhisa
Graduate School of Pharmaceutical Sciences, Nagoya City University
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KONDO Toshiya
Faculty of Pharmaceutical Sciences, Kitasato University
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Iida K
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Mizukami Hajime
Faculty Of Pharmaceutical Sciences Nagoya City University
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Mizukami H
Dep. Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City Univ.
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IIDA Katsuhisa
Faculty of Pharmaceutical Sciences, Nagoya City University
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Mizukami Hajime
Faculty Of Pharmaceutical Science Nagoya City University
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