An Elicitor-Inducible NADP-Malic Enzyme in Lithospermum erythrorhizon Cultured Cells : cDNA Cloning and Characterization
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概要
- 論文の詳細を見る
The differential display technique was used to isolate genes expressed during yeast extract-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures, and a cDNA clone whose deduced amino acid sequence is similar to plant cytosolic NADP-malic enzymes was identified and designated LeME. The heterologously-expressed LeME oxidized L-malate in presence of NADP, but it did not catalyze formation of 4-hydroxyphenyllactic acid from 4-hydroxyphenylpyruvate in presence of NADPH, one of the crucial biosynthetic steps leading to rosmarinic acid. The potential role of LeME in elicitor-inducible rosmarinic acid biosynthesis is discussed.
- 日本植物細胞分子生物学会の論文
- 2002-06-01
著者
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荻原 幸夫
名城大学臨床漢方治療学研究室
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MIZUKAMI Hajime
Graduate School of Pharmaceutical Sciences, Nagoyacity University
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OGIHARA Yukio
Nihon Pharmaceutical University
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Ogihara Y
Nihon Pharmaceutical University
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Ogihara Yukio
Laboratory Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City University
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Ogihara Yukio
Faculty Of Pharmacy Meijo Universitv
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Ogihara Yukio
Department Of Pharmacognosy Faculty Of Pharmaceutical Sciences Nagoya City University
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Ogihara Yukio
Graduate School Of Pharmaceutical Sciences Nagoya City University
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MATSUNO Michiyo
Graduate School of Pharmaceutical Sciences, Nagoya City University
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Mizukami Hajime
Department Of Legal Medicine Asahikawa Medical College
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Mizukami Hajime
Graduate School Of Pharmaceutical Sciences Nagoyacity University
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Mizukami Hajime
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Matsuno M
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Matsuno Michiyo
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Mizukami H
Dep. Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City Univ.
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荻原 幸夫
名古屋市立大学薬学部
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