Sequence Analysis of Chloroplast chlB Gene of Medicinal Ephedra Species and Its Application to Authentication of Ephedra Herb(Pharmacognosy)
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概要
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Chloroplast chlB gene encoding subunit B of light-independent protochlorophyllide reductase was amplified from herbarium and crude drug specimens of Ephedra sinica, E. intermedia, E, equisetina, and E. przewalskii. Sequence comparison of the chlB gene indicated that all the E. sinica specimens have the same sequence type (Type S) distinctive from other species, while there are two sequence types (Type E1 and Type E2) in E. equisetina. E. intermedia and E. prezewalskii revealed an identical sequence type (Type IP). E. sinica was also identified by digesting the chlB fragment with Bcl I. A novel method for DNA authentication of Ephedra Herb based on the sequences of the chloroplast chlB gene and internal transcribed spacer of nuclear rRNA genes was developed and successfully applied for identification of the crude drugs obtained in the Chinese market.
- 公益社団法人日本薬学会の論文
- 2006-06-01
著者
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MIZUKAMI Hajime
Graduate School of Pharmaceutical Sciences, Nagoyacity University
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Oba Koji
Research And Development Department Asgen Pharmaceutical Co. Ltd.
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Sekita Setsuko
Tsukuba Medicinal Plant Research Station National Institute Of Health Sciences
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GUO Yahong
Graduate School of Pharmaceutical Sciences, Nagoya City University
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Guo Yahong
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Iwai Kasumi
Research And Development Department Asgen Pharmaceutical Co. Ltd.
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Mizukami Hajime
Graduate School Of Pharmaceutical Sciences Nagoya City University
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TSURUGA Ayako
Graduate School of Pharmaceutical Sciences, Nagoya City University
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YAMAGUCHI Shigeharu
Research and Development Department, Asgen Pharmaceutical Co., Ltd.
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Tsuruga Ayako
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Yamaguchi Shigeharu
Research And Development Department Asgen Pharmaceutical Co. Ltd.
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