Cloning and Bacterial Expression of Karasurins, Ribosome-Inactivating Proteins (RIPs) from Trichosanthes Kirilowii var. Japonica

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概要

• 論文の詳細を見る

• A genomic clone encoding a karasurin precursor was isolated from root tubers of Trichosanthes kirilowii var. japonica. The sequence analysis indicates that karasurin-A and karasurin-C exhibiting abortifacient and ribosome-inhibiting activities are produced from a common karasurin precursor by proteolitically cleaving at a different site. Northern hybridization analysis shows that the karasurin gene was expressed only in root tubers. An efficient system to overproduce the recombinant karasurin-A as fused protein with the maltose-binding protein was established.

• 日本植物細胞分子生物学会の論文
• 2001-12-01

著者

• MIZUKAMI Hajime

  Graduate School of Pharmaceutical Sciences, Nagoyacity University

• 近藤 俊哉

  Faculty Of Pharmaceutical Sciences Nagoya City University

• OGIHARA Yukio

  Nihon Pharmaceutical University

• Kondo T

  Research Division The Green Cross Corporation

• Ogihara Y

  Nihon Pharmaceutical University

• Ogihara Yukio

  Laboratory Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City University

• Ogihara Yukio

  Faculty Of Pharmacy Meijo Universitv

• Ogihara Yukio

  Graduate School Of Pharmaceutical Sciences Nagoya City University

• IIDA Katsuhisa

  Graduate School of Pharmaceutical Sciences, Nagoya City University

• KONDO Toshiya

  Faculty of Pharmaceutical Sciences, Kitasato University

• YOSHIKAWA Takafumi

  Faculty of Pharmaceutical Sciences, Kitasato University

• Iida K

  Graduate School Of Pharmaceutical Sciences Nagoya City University

• Mizukami Hajime

  Department Of Legal Medicine Asahikawa Medical College

• Mizukami Hajime

  Graduate School Of Pharmaceutical Sciences Nagoya City University

• Yoshikawa Takafumi

  Faculty Of Pharmaceutical Sciences Kitasato University

• Mizukami H

  Dep. Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City Univ.

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