Cloning and Bacterial Expression of Karasurins, Ribosome-Inactivating Proteins (RIPs) from Trichosanthes Kirilowii var. Japonica
スポンサーリンク
概要
- 論文の詳細を見る
A genomic clone encoding a karasurin precursor was isolated from root tubers of Trichosanthes kirilowii var. japonica. The sequence analysis indicates that karasurin-A and karasurin-C exhibiting abortifacient and ribosome-inhibiting activities are produced from a common karasurin precursor by proteolitically cleaving at a different site. Northern hybridization analysis shows that the karasurin gene was expressed only in root tubers. An efficient system to overproduce the recombinant karasurin-A as fused protein with the maltose-binding protein was established.
- 日本植物細胞分子生物学会の論文
- 2001-12-01
著者
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MIZUKAMI Hajime
Graduate School of Pharmaceutical Sciences, Nagoyacity University
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近藤 俊哉
Faculty Of Pharmaceutical Sciences Nagoya City University
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OGIHARA Yukio
Nihon Pharmaceutical University
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Kondo T
Research Division The Green Cross Corporation
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Ogihara Y
Nihon Pharmaceutical University
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Ogihara Yukio
Laboratory Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City University
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Ogihara Yukio
Faculty Of Pharmacy Meijo Universitv
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Ogihara Yukio
Graduate School Of Pharmaceutical Sciences Nagoya City University
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IIDA Katsuhisa
Graduate School of Pharmaceutical Sciences, Nagoya City University
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KONDO Toshiya
Faculty of Pharmaceutical Sciences, Kitasato University
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YOSHIKAWA Takafumi
Faculty of Pharmaceutical Sciences, Kitasato University
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Iida K
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Mizukami Hajime
Department Of Legal Medicine Asahikawa Medical College
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Mizukami Hajime
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Yoshikawa Takafumi
Faculty Of Pharmaceutical Sciences Kitasato University
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Mizukami H
Dep. Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Nagoya City Univ.
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