Quantitative Determination of Carthamin in Carthamus Red by 1H-NMR Spectroscopy
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概要
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Carthamus Red is a food colorant prepared from the petals of Carthamus tinctorius (Asteraceae) whose major pigment is carthamin. Since an authentic carthamin standard is difficult to obtain commercially for the preparation of calibration curves in HPLC assays, we applied 1H-NMR spectroscopy to the quantitative determination of carthamin in commercial preparations of Carthamus Red. Carthamus Red was repeatedly extracted in methanol and the extract was dissolved in pyridine-d5 containing hexamethyldisilane (HMD) prior to 1H-NMR spectroscopic analysis. The carthamin contents were calculated from the ratios of singlet signal intensities at approximately σ: 9.3 derived from H-16 of carthamin to those of the HMD signal at σ: 0. The integral ratios exhibited good repeatability among NMR spectroscopic analyses. Both the intra-day and inter-day assay variations had coefficients of variation of <5%. Based on the coefficient of absorption, the carthamin contents of commercial preparations determined by 1H-NMR spectroscopy correlated well with those determined by colorimetry, although the latter were always approximately 1.3-fold higher than the former, irrespective of the Carthamus Red preparations. In conclusion, the quantitative 1H-NMR spectroscopy used in the present study is simple and rapid, requiring no carthamin standard for calibration. After HMD concentration has been corrected using certified reference materials, the carthamin contents determined by 1H-NMR spectroscopy are System of Units (SI)-traceable.
- 公益社団法人 日本薬学会の論文
著者
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AKIYAMA Hiroshi
National Institute of Health Sciences
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SUGIMOTO Naoki
National Institute of Health Sciences
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Mizukami Hajime
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Terasaka Kazuyoshi
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Kato Setsuko
Graduate School Of Pharmaceutical Sciences Nagoya City University
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YOSHIDA Takamitsu
Graduate School of Pharmaceutical Sciences, Nagoya City University
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Bai Fan
Graduate School Of Frontier Biosciences Osaka University:clarendon Laboratory Department Of Physics
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Akiyama Hiroshi
National Food Research Institute National Agriculture And Food Research Organization
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Yamazaki Takeshi
Faculty Of Integrated Arts And Sciences Hiroshima University
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Yoshida Takamitsu
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Bai Fan
Graduate School of Pharmaceutical Sciences, Nagoya City University
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Yamazaki Takeshi
Faculty of Human Life Sciences, Jissen Women's University
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