Real-Time PCR Method Using Capturing Oligo-Immobilized PCR Tubes to Determine the Specific Gene for Soybean and Genetically Modified Soybean in Food Matrices
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概要
- 論文の詳細を見る
A new real-time PCR method using capturing oligo-immobilized PCR tubes is described. This method was used to detect specific genes for soybean and genetically modified (GM) soybean in food matrices. In a standard reaction using soybean genomic DNA and a capturing oligo for the lectin gene (Le1) immobilized on the tube, we examined the effects of such hybridization conditions as the location, length, and amount of the capturing oligo, and the incubation time and temperature. Under optimized conditions, the copy number of Le1 was determined in a concentration-dependent manner from soybean genomic DNA and soybean lysate (DNA 10–1000 ng, r=0.99; lysate 1–100%, r=0.99). The copy number of a Roundup Ready soybean (RRS) gene was also successfully detected in a concentration-dependent manner (1–100%, r=0.99) from GM soybean lysate, using PCR tubes with an immobilized capturing oligo for the transgene. Our data indicate that this is a rapid and simple method to determine specific genes for soybean and GM soybean in food matrices.
- 社団法人 日本農芸化学会の論文
- 2008-11-23
著者
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TESHIMA Reiko
National Institute of Health Sciences
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KONDO Kazunari
National Institute of Health Sciences
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AKIYAMA Hiroshi
National Institute of Health Sciences
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Teshima Reiko
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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Teshima R
National Inst. Of Health Sciences
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Teshima Reiko
国立医薬品食品衛生研究所 機能生化学部
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Teshima Reiko
名古屋市立大学 薬学研究科
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Teshima Reiko
National Institute Of Health Science
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Kondo Kazunari
National Inst. Of Health Sciences
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KITTA Kazumi
National Food Research Institute
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Saito Shin
Sumitomo Bakelite Co., Ltd.
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Kinoshita Kenji
School of Pharmaceutical Sciences, Mukogawa Women's University
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Harikai Naoki
School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women’s University
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Saito Shin
S-BIO Development Department, Sumitomo Bakelite Co.
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Abe Midori
S-BIO Development Department, Sumitomo Bakelite Co.
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Abe Midori
Sumitomo Bakelite Co.
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Kitta Kazumi
National Food Res. Inst.
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Sakai Shinobu
National Inst. Of Health Sciences
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Teshima Reiko
National Inst. Of Health Sciences
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Harikai Naoki
School Of Pharmaceutical Sciences Mukogawa Women's University
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Sakai Shinobu
Sumitomo Bakelite Co. Ltd.
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Sakai Shinobu
Research Associate Department Of Architectural Engineering Hokkaido University
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Kinoshita Kenji
School Of Pharmaceutical Sciences Mukogawa Women's University
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Akiyama H
Real Estate Science Graduate School Of Science And Technology Nihon University
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Akiyama Hiroshi
Division Of Novel Foods And Immunochemistry National Institute Of Health Science
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Akiyama Hiroshi
National Food Research Institute National Agriculture And Food Research Organization
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Saito Shin
Sumitomo Bakelite Co. Ltd.
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Harikai Naoki
School Of Pharmacy And Pharmaceutical Sciences Mukogawa Women's University
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Kondo Kazunari
National Food Research Institute, National Agriculture and Food Research Organization
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Harikai Naoki
School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University
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