Development and Interlaboratory Validation of Quantitative Polymerase Chain Reaction Method for Screening Analysis of Genetically Modified Soybeans
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概要
- 論文の詳細を見る
A novel real-time polymerase chain reaction (PCR)-based quantitative screening method was developed for three genetically modified soybeans: RRS, A2704-12, and MON89788. The 35S promoter (P35S) of cauliflower mosaic virus is introduced into RRS and A2704-12 but not MON89788. We then designed a screening method comprised of the combination of the quantification of P35S and the event-specific quantification of MON89788. The conversion factor (Cf) required to convert the amount of a genetically modified organism (GMO) from a copy number ratio to a weight ratio was determined experimentally. The trueness and precision were evaluated as the bias and reproducibility of relative standard deviation (RSDR), respectively. The determined RSDR values for the method were less than 25% for both targets. We consider that the developed method would be suitable for the simple detection and approximate quantification of GMO.
著者
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Futo Satoshi
Fasmac Co. Ltd.
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Mano Junichi
National Food Research Institute
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Kitta Kazumi
National Food Res. Inst.
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Teshima Reiko
National Inst. Of Health Sciences
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Furui Satoshi
National Food Res. Inst.
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Akiyama Hiroshi
National Food Research Institute National Agriculture And Food Research Organization
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Minegishi Yasutaka
Nippon Gene Co. Ltd.
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Takabatake Reona
National Food Research Institute
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Furui Satoshi
National Food Research Institute, National Agriculture and Food Research Organization
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Koiwa Tomohiro
Food and Agricultural Materials Inspection Center
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Kurashima Takeyo
National Food Research Institute, National Agriculture and Food Research Organization
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Onishi Mari
FASMAC Co., Ltd.
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