Bi-directional Relationship of in Vitro Mast Cell-Nerve Communication Observed by Confocal Laser Scanning Microscopy

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Communication between nerves and mast cells is a protitypic demonstration of neuro-immune interaction. Recently, we used an i vitro co-culture approach comprising cultured murine superior cervical ganglia(SCG) and rat basophilic leukimia(RBL)cells to study this interaction. Previously, we concentrated mainly on the activation signal from neurtes to mast cells(RBL). However, it is proposed that mast cell-nerve communication is not a one-sided relationship but a bi-derectional one. In the present work, we studied the communication from mast cells to neurites. We observed that binding of anti-IgE receptor antibodies to mast cells increases calcium ion concentration [Ca^<2+>]_i in SCG neurtes. This indicates that [Ca^<2+>]_i in neurites increased after an increase of [Ca^<2+>]_i in mast cells. The lag-time of neurite activation was several times longer than that of mast cell activation. The correlation coefficient between the lag-times for mast cell and nerve activation was calculated to be 0.81. In addition, the fluorescence images showed that calcium signals i SCG neurites were able to extend to a long distance(100-200μm)from the site where mast cells(RBL)attached to neurites.
公益社団法人日本薬学会の論文
2001-03-01