Method for Delivering Radiolabeled Single-Chain Fv Antibody to the Brain
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概要
- 論文の詳細を見る
Radiolabeled antibody has attractive features as a therapeutic agent or diagnostic reagent. However, it is difficult for radiolabeled antibody to enter the central nervous system (CNS). The purpose of this study was to develop a method of delivering radiolabeled single-chain Fv (scFv) antibody into the CNS with the transactivator of transcription (TAT), one of the protein transduction domains. Oligonucleotide encoding TAT was linked to the 5'-terminal of the scFv gene. The construct was subcloned into the pET-23b vector, and the recombinant protein was expressed as an inclusion body in Escherichia coli. After solubilization and purification, the recombinant protein was oxidatively labeled with ^<125>I, and the radiolabeled recombinant protein was injected intraperitoneally into mice. Six hours later the brains were collected and homogenized, and the protein fractions were prepared by acetone precipitation. The radioactivity in the cerebrum was about 1.6-fold higher in mice administered TAT-scFv than in those administered scFv alone. The radiolabeled TAT-scFv antibody was delivered into the cerebrum more efficiently than radiolabeled scFv antibody without TAT, suggesting that TAT peptide could be a candidate tool for delivering radiolabeled scFv antibody into the CNS.
- 社団法人日本薬学会の論文
- 2004-04-01
著者
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TESHIMA Reiko
National Institute of Health Sciences
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Sawada Junichi
Project Team For Pharmacogenetics National Institute Of Health Sciences
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SAWADA Jun-ichi
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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HACHISUKA Akiko
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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NAKAJIMA Osamu
Division of Biochemistry and Immunochemistry National Institute of Health Sciences
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Hachisuka Akiko
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences
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Hachisuka Akiko
国立医薬品食品衛生研究所
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Hachisuka Akiko
National Institute Of Health Sciences
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中島 修
東京大学分子細胞生物学研究所
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Teshima Reiko
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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Okunuki Haruyo
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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Takagi Kayoko
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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Takagi Kayoko
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences (nihs)
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Takagi Kayoko
国立医薬品食品衛生研究所 機能生化学部
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Takagi Kayoko
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences
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Okunuki Haruyo
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences
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Okunuki Haruyo
国立医薬品食品衛生研究所 機能生化学部
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Teshima R
National Inst. Of Health Sciences
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Teshima Reiko
国立医薬品食品衛生研究所 機能生化学部
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Teshima Reiko
名古屋市立大学 薬学研究科
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Sawada Jun-ichi
Division Of Functional Biochemistry And Genomics National Institute Of Health Sciences
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Sawada Jun-ichi
Division Of Biochemistry And Immunochemistry Natinal Institute Of Health Sciences
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Teshima Reiko
National Institute Of Health Science
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Teshima Reiko
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences
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Nakajima Osamu
National Inst. Of Health Sciences
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Sawada J
Mie Univ. School Of Medicine Mie Jpn
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Takagi K
National Inst. Of Health Sci. Tokyo Jpn
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Sawada J
National Inst. Of Health Sci. Tokyo Jpn
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