Comparative Study of in Vitro Digestibility of Food Proteins and Effect of Preheating on the Digestion
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概要
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Information on the comparative digestibility of food allergens and non-allergenic proteins is crucial when stability to digestion is to be used as a criterion to assess the allergenic potential of novel proteins. Preheating ef fect on in vitro digestibility has not been fully examined. In this study we investigated the preheating effect of in vitro digestibility of several proteins and their proteolytic fragments in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). Five major food allergens, ovalbumin (OVA), ovomucoid (OVM),β-lactoglobulin (BLG), bovine serum albumin (BSA), soybean trypsin inhibitor (STI), four proteins of unproven allergenicity, horseradish peroxidase (HRP), ribulose-l,5-bisphosphate carboxylase/oxidase (RBC), phosphinothricin acetyl transferase (PAT) and zein from corn, and plant lectin, concanavalin A (Con A) were preheated (at IOO°C for 5 min) or not preheated, and then digested in SGF or SIF. Food allergens were relatively stable in both SGF and SIF. Among the allergens, digestibility of OVA in both SGF and SIF was markedly decreased, and BLG and STI were relatively stable after preheating. Digestibility of ConA in SGF and SIF was markedly decreased by preheating. Digestibility of non-allergenic proteins in SGF was higher than the allergenic proteins. From these results, because of the marked increase of the digestibility in several proteins by preheating, systematic information concerning the effect of food treatment on protein digestion is necessary to assess the relationship between allergenic potential and the digestibility of food protein.
- 公益社団法人日本薬学会の論文
- 2003-07-01
著者
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TESHIMA Reiko
National Institute of Health Sciences
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Sawada Junichi
Project Team For Pharmacogenetics National Institute Of Health Sciences
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Sawada Jun-ichi
National Institute Of Health Sciences
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Teshima Reiko
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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Okunuki Haruyo
Division of Biochemistry and Immunochemistry, National Institute of Health Sciences
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Takagi Kayoko
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences (nihs)
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Takagi Kayoko
国立医薬品食品衛生研究所 機能生化学部
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Takagi Kayoko
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences
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Okunuki Haruyo
Division Of Biochemistry And Immunochemistry National Institute Of Health Sciences
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Okunuki Haruyo
国立医薬品食品衛生研究所 機能生化学部
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Teshima R
National Inst. Of Health Sciences
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Teshima Reiko
国立医薬品食品衛生研究所 機能生化学部
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Teshima Reiko
名古屋市立大学 薬学研究科
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Teshima Reiko
National Institute Of Health Science
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TAKAGI Kayoko
National Institute of Health Sciences
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OKUNUKI Haruyo
National Institute of Health Sciences
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Teshima Reiko
National Inst. Of Health Sciences
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Sawada J
Mie Univ. School Of Medicine Mie Jpn
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Takagi K
National Inst. Of Health Sci. Tokyo Jpn
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Sawada J
National Inst. Of Health Sci. Tokyo Jpn
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