The Hyperresponsiveness of W/W^v Mice to Oral Sensitization Is Associated with a Decrease in TCRγδ-T Cells(Biochemistry/Molecular Biology)

概要

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We have already reported that WBB6F1-W/W^v (W/W^v) mice, which have mutations in the c-kit gene, are highly susceptible to oral sensitization, and that the proportion of TCRγδ-T cells among the intraepithelial lymphocytes (IELs)(γδ-IELs) of W/W^v is much lower than in congenic wild-type (+/+) mice. In this study we examined an inhibitory role of γδ-IELs in oral sensitization using two different methods. First, wild-type (+/+) mice were sensitized by oral administration of 1.0 mg ovalbumin (OVA) by gavage every day for 9 weeks after anti-TCRγδ antibody treatment 4 times. The treatment resulted in an enhanced OVA-specific IgGl antibody production, active systemic anaphylaxis (ASA), and Th2-dominant cytokine production. Next, W/W^v mice whose bone marrow cells were reconstituted from C57BL/6J mice for 5 months were sensitized by oral administration of OVA. The OVA-specific IgGl antibody titer in the bone marrow-reconstituted W/W^v mice was neither significantly enhanced, nor ASA was induced. The proportion of γδ-IELs in the reconstituted mice was much higher than that in the untreated W/W^v mice. The above findings suggest that the decrease or increase in number of γδ-IELs enhances or decreases oral sensitization respectively. These results show that γδ-IELs have an important role in the oral tolerance to food antigens.
社団法人日本薬学会の論文
2005-04-01