Quantification of Genetically Modified Soybeans Using a Combination of a Capillary-Type Real-Time PCR System and a Plasmid Reference Standard
スポンサーリンク
概要
- 論文の詳細を見る
Because the labeling of grains and feed- and foodstuffs is mandatory if the genetically modified organism (GMO) content exceeds a certain level of approved genetically modified varieties in many countries, there is a need for a rapid and useful method of GMO quantification in food samples. In this study, a rapid detection system was developed for Roundup Ready® Soybean (RRS) quantification using a combination of a capillary-type real-time PCR system, a LightCycler® real-time PCR system, and plasmid DNA as the reference standard. In addition, we showed for the first time that the plasmid and genomic DNA should be similar in the established detection system because the PCR efficiencies of using plasmid DNA and using genomic DNA were not significantly different. The conversion factor (Cf) to calculate RRS content (%) was further determined from the average value analyzed in three laboratories. The accuracy and reproducibility of this system for RRS quantification at a level of 5.0% were within a range from 4.46 to 5.07% for RRS content and within a range from 2.0% to 7.0% for the relative standard deviation (RSD) value, respectively. This system rapidly monitored the labeling system and had allowable levels of accuracy and precision.
- 社団法人 日本農芸化学会の論文
- 2006-04-23
著者
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Maitani Tamio
国立医薬品食品衛生研究所
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Maitani Tamio
National Institute of Health Sciences
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AKIYAMA Hiroshi
National Institute of Health Sciences
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Maitani Tamio
Division of Foods, National Institute of Health Sciences
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HINO Akihiro
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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WATANABE Takahiro
National Institute of Health Sciences
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Maitani T
Division Of Foods National Institute Of Health Sciences
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TOYOTA Akie
Hiroshima Prefectural Institute of Public Health and Environment
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SUGIMURA Mitsunori
Hiroshima Prefectural Institute of Public Health and Environment
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ESAKA Muneharu
Graduate School of Biosphere Sciences, Hiroshima University
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KIKUCHI Hiroyuki
National Institute of Health Sciences
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KANAMORI Hisayuki
Hiroshima Prefectural Institute of Public Health and Environment
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Machiguchi Takahisa
Faculty Of Science Saitama University
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Maitani Tamio
National Institute For Environmental Studies
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Toyota Akie
Hiroshima Prefectural Institute For Public Health And Environment Science
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米谷 民雄
Division Of Foods National Institute Of Health Sciences
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Kawakami Hiroshi
Dep. Of Food Sci. & Nutrition Kyoritsu Women's Univ.
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Hino Akihiro
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
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Akiyama H
Real Estate Science Graduate School Of Science And Technology Nihon University
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Esaka Muneharu
Graduate School Of Biosphere Science Hiroshima University
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Akiyama Hiroshi
Division Of Novel Foods And Immunochemistry National Institute Of Health Science
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Kanamori Hisayuki
Hiroshima Prefectural Health Environment Center
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Akiyama Hiroshi
National Food Research Institute National Agriculture And Food Research Organization
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HINO Akihiro
National Food Research Institute (NFRI), National Agriculture and Food Research Organization (NARO)
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