A Real-Time Quantitative PCR Detection Method for Pork, Chicken, Beef, Mutton, and Horseflesh in Foods
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概要
- 論文の詳細を見る
A rapid real-time quantitative PCR method to detect trace amounts of pork, chicken, beef, mutton, and horseflesh in foods was developed. The primers and TaqMan MGB (minor groove binder) probes were designed on the gene encoding cytochrome b for the specific detection of each species. The limit of quantification of this method was found to be 100 fg/μl of each mitochondrial DNA in 10 ng/μl of the wheat mitochondrial DNA matrix. The calculated R2 values of the standard curves for the five species ranged between 0.994 and 0.999. This method would be particularly useful in the detection of hidden meat mince in processed foods, which would verify food labeling and gain consumers’ trust.
- 社団法人 日本農芸化学会の論文
- 2007-12-23
著者
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AKIYAMA Hiroshi
National Institute of Health Sciences
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佐藤 雅彦
伊藤ハム株式会社中央研究所
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AKIYAMA Hiroshi
Division of Food, National Institute of Health Sciences
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佐藤 雅彦
伊藤ハム・中央研究所
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TANABE Soichi
Graduate School of Biosphere Science Hiroshima University
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Akiyama H
Real Estate Science Graduate School Of Science And Technology Nihon University
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Tanabe Soichi
Graduate School Of Biosphere Sci. Hiroshima Univ.
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Tanabe Soichi
Hiroshima University Graduate School Of Biosphere Science
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Akiyama Hiroshi
Division Of Novel Foods And Immunochemistry National Institute Of Health Science
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Sato Masahiko
Central Research Institute Itoham Foods Inc.
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Fujimura Tatsuya
Res. And Dev. Center Nippon Meat Packers Inc.
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Yano Takeo
Nagahama Institute For Biochemical Science Oriental Yeast Co. Ltd.
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Yano Takeo
Biochemical Production And Development Center Nagahama Branch Oriental Yeast Co. Ltd.
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SATO Masahiko
Itoham Foods Inc.
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HASE Makiko
Application Support Department, Applied Biosystems Japan
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FUJIMURA Tatsuya
R&D Center, Nippon Meat Packers, Inc.
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Hase Makiko
Application Support Department Applied Biosystems Japan
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Akiyama Hiroshi
National Food Research Institute National Agriculture And Food Research Organization
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Sato Masahiko
Ito ham Corp.
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佐藤 雅彦
伊藤ハム株式会社 中央研究所
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