Formation of Zinc Protoporphyrin IX from Myoglobin in Porcine Heart Extract
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概要
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The formation of zinc protoporphyrin IX (ZPP) in porcine heart extract was investigated by visible absorption and fluorescent spectral analysis. Characteristic absorption peaks of ZPP were observed at 417, 546, and 584nm after anaerobic incubation of the extract with metmyoglobin and ZnCl2 in the dark at 30°C for 72h. In the fluorescent spectra (excitation : 410nm), a strong peak due to ZPP was obtained at 589nm. The time-course study showed that ZPP formation greatly increased after a lag period, during which metmyoglobin was reduced. ZPP formation was significantly facilitated by the use of oxymyoglobin. Zinc chelatase activity in the extract was estimated as 138mU/mL-extract and its specific activity was 2.95mU/mg-protein. The difference in activities with aerobic and anaerobic incubation was not significant. Measurements at pH 5.5-7.0 showed that activities were higher at lower pH. The activity of zinc chelatase was stimulated by the presence of ATP, and the highest activity was obtained at an ATP concentration of 2.5mg/mL.
- 社団法人 日本食品科学工学会の論文
- 2006-05-01
著者
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ISHIKAWA Hiroya
Department of Polymer Science and Engineering, Faculty of Textile Science, Kyoto Institute of Techno
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佐藤 雅彦
伊藤ハム株式会社中央研究所
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佐藤 雅彦
伊藤ハム・中央研究所
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Sato Masahiko
Central Research Institute Itoham Foods Inc.
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Matsumoto Kiyoshi
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Of Kyushu Universi
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Matsumoto Kiyoshi
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
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KAWABUCHI Taiki
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu Univer
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SATO Masahiko
Itoham Foods Inc.
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NUMATA Masahiro
Itoham Foods Inc.
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YOSHIHARA Miyuki
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu Univer
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BABA Ai
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu Univer
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Baba Ai
Faculty Of Agriculture Graduate School Kyushu University
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Kawabuchi Taiki
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Of Kyushu Universi
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Ishikawa Hiroya
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Of Kyushu Universi
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Ishikawa Hiroya
Department Of Bioscience And Biotechnology Faculty Of Agriculture Graduate School Kyushu University
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Yoshihara Miyuki
Faculty of Agriculture, Graduate School, Kyushu University
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Yoshihara Miyuki
Faculty Of Agriculture Graduate School Kyushu University
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Sato Masahiko
Ito ham Corp.
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佐藤 雅彦
伊藤ハム株式会社 中央研究所
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