Flow Injection Analysis of Lactose in Milk Using a Chemically Modified Lactose Electrode
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概要
- 論文の詳細を見る
β-Galactosidase and glucose oxidase were immobilized with bovine serum albumin using glutaraldehyde on to a glassy carbon electrode silanized with 3-aminopropyltriethoxysilane. The laboratory-constructed lactose electrode was used for flow injection analysis to determine the lactose content in milk. Electrochemical interference could be detected by a non-enzymatic electrode (W2) and the current was subtracted from that of the enzymatic electrode (W1), providing an accurate measurement of the hydrogen peroxide that was enzymatically generated. The peak current was linearly related to the lactose concentration in the range 10-4-1.5 × 10-3M (original concentration) and 40 samples/hr could be analyzed. The relative standard deviation for 10 assays was less than 2%. The proposed method was compared with the chloramine T method and the values determined by both methods were in good agreement.
- 社団法人 日本農芸化学会の論文
著者
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Osajima Yutaka
Department Of Bioresources And Biosciences Hiroshima Prefectural University
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Ukeda Hiroyuki
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Matsumoto Kiyoshi
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
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HAMADA Osamu
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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