Inactivation of Cu, Zn-Superoxide Dismutase by Intermediates of Maillard Reaction and Glycolytic Pathway and Some Sugars
スポンサーリンク
概要
- 論文の詳細を見る
Human Cu, Zn-superoxide dismutase (SOD) was incubated with various intermediates of the Maillard reaction and glycolytic pathway (arabinose, glyoxal, glycolaldehyde, glyceraldehyde, glyceraldehyde 3-phosphate, and dihydroxyacetone) and some reducing sugars (sorbose, xylose, and ribose). The change of the activity and the molecular weight were measured and compared with that of SOD incubated with glucose or fructose. Sorbose, xylose, and ribose decreased the activity with a rate comparable to fructose. Site-specific and random fragmentation were observed upon the incubation with them. Arabinose showed a similar inactivation rate as glucose. The intermediates other than arabinose had a high inactivation rate. Especially, glyceraldehyde, glycolaldehyde, and glyoxal most strongly lowered the activity in a concentration-dependent manner and a significant inactivation was recognized even at 1 mM level. SDS-PAGE band patterns indicated that the inactivation by those carbonyl compounds occurred by both crosslinking and site-specific fragmentation of SOD.
- 社団法人日本農芸化学会の論文
- 1997-12-23
著者
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SAWAMURA Masayoshi
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Sawamura M
Kochi Univ. Nankoku Jpn
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Sawamura M
Kochi Univ. Kochi Jpn
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Sawamura Masayoshi
Department Of Agricultural Chemistry Faculty F Agriculture Kochi University
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UKEDA Hiroyuki
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Sawamura M
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Ukeda H
Kochi Univ. Nankoku Jpn
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Ukeda Hiroyuki
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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HASEGAWA Yuko
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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ISHI Toshinao
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Harada Y
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Ishi Toshinao
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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