A New Approach to the Co-immobilization of Alcohol Dehydrogenase and NAD on Glutaraldehyde-activated Sepharose and Its Application to the Enzymatic Analysis of Ethanol
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概要
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A new method is described for the co-immobilization of enzyme alcohol dehydrogenase (ADH) and coenzyme NAD onto Sepharose gel. In order to increase the degree of freedom of NAD, spacer molecules were introduced to the support by allowing it to repeatedly react with hexamethylenediamine and glutaraldehyde, and ADH and NAD were co-immobilized to the spacer-modified Sepharose. The results suggest that the re-usability of Sepharose-bound ADH and NAD (AN-Seph) depended both on the concentration and the reaction time of glutaraldehyde to a great extent. Furthermore, it is suggested that AN-Seph prepared under the optimum conditions might be applicable to the determination of ethanol.
- 社団法人 日本農芸化学会の論文
著者
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MATSUMOTO Kiyoshi
Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu Universi
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Matsumoto K
Division Of Bioscience And Bioenvironmental Sciences Faculty Of Agriculture Graduate School Of Kyush
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OSAJIMA Yutaka
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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Osajima Yutaka
Department Of Bioresources And Biosciences Hiroshima Prefectural University
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UKEDA Hiroyuki
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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KAMIKADO Hideaki
Department of Food Science and Technology, Faculty of Agriculture, Kyushu University
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Ukeda Hiroyuki
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Matsumoto Kiyoshi
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
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