Thin-layer Flow Cell System for Ascorbate Enzyme Electrode
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概要
- 論文の詳細を見る
A thin-layer flow cell system for the determination of L-ascorbic acid by an ascorbate electrode was constructed and several components of this system were investigated. The most preferable conditions for optimum operation of the system were as follows: injection volume 150μl, delay coil length 60cm, flow rate 1 ml/min, temperature 20°C, cell spacer thickness 0.2mm. The linear response region was 0.2-3.0 HIM and 0.02-0.5 HIM (original L-ascorbic acid concentration) in the cases of pure oxygen and atmospheric oxygen bubbling, respectively. The relative standard variation at 1.5mM L-ascorbic acid was 3.1% for 20 successive assays. The measuring time was 2-3 min for each of these assays.
- 社団法人 日本農芸化学会の論文
著者
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Osajima Yutaka
Department Of Bioresources And Biosciences Hiroshima Prefectural University
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Matsumoto Kiyoshi
Department Of Applied Chemistry Faculty Of Engineering Kanagawa University
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NAOTSUKA Masaaki
Department of Food Science and Technology, Faculty of Agriculture, 46-09, Kyushu University
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SHIRASAKA Yoshihisa
Department of Food Science and Technology, Faculty of Agriculture, 46-09, Kyushu University
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NOMURA Takakazu
Department of Food Science and Technology, Faculty of Agriculture, 46-09, Kyushu University
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