Determination of Glycerol in Wine by Amperometric Flow Injection Analysis with an Immobilized Glycerol Dehydrogenase Reactor(Analytical Chemistry)

概要

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A glycerol sensor, in which glycerol dehydrogenase was immobilized on Amino-Cellulofine, was developed for the continuous determination of glycerol in wine. NADH produced by the enzyme reaction was monitored amperometrically with a platinum electrode. A sandwich method was used for sample injection as an effective mixing mode of NAD and sample. A typical calibration curve showed a slightly convex curve between response and glycerol concentration (0.1-2 mM; 1.8×10^<-8>-3.6×10^<-7> mol). The relative standard deviation for ten measurements of 9×10^<-8> mol glycerol was 0.5%. The proposed method was used for wine analysis and was compared with an F-kit (enzymatic, spectrophotometric) method.
社団法人日本農芸化学会の論文
1991-04-23