Evaluation of α-Glucosidase Inhibition by Using an Immobilized Assay System
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概要
- 論文の詳細を見る
The inhibitory effects of natural and synthetic inhibitors on the intestinal membrane-bound hydrolase, α-glucosidase (AGH), were evaluated by using an immobilized cyanogen bromide-activated Sepharose 4B support. Immobilized AGH (iAGH) inhibition study by synthetic inhibitors (acarbose and voglibose) revealed that the magnitude of inhibition differed from that in the free AGH (fAGH) study : IC_<50> value of acarbose in iAGH-maltase assay system, 340-430 nM; fAGH, 11 nM. iAGH-maltase inhibition by both inhibitors was influenced by blocking reagents with different functional groups (COOH, OH, CH_3,and NH_2 groups). On the other hand, significant iAGH-sucrase inhibitory activity was observed only when using the negatively charged support induced by 0.1 M β-alanine. The K_m values obtained in the iAGH assay system were similar to those from the fAGH method. With natural inhibitors, the iAGH-sucrase inhibitory activity of D-xylose, with in vivo glucose suppression, increased twice compared to that in fAGH. Green tea extract gave almost the same inhibition for both AGH assay systems.
- 公益社団法人日本薬学会の論文
- 2000-09-01
著者
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Matsumoto K
Division Of Bioscience And Bioenvironmental Sciences Faculty Of Agriculture Graduate School Of Kyush
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Oki Tomoyuki
Division Of Bioresource And Bioenvironmental Sciences Faculty Of Agriculture Graduate School Kyushu
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MATSUMOTO Kenji
Gifu International Institute of Biotechnology
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Matsui T
Kyushu Univ. Fukuoka Jpn
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Matsui Toshiro
Fac. Of Agriculture Graduate School Of Kyushu Univ.
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MATSUMOTO Kiyoshi
Division of Toxicology, National Institute of Hygienic Sciences
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MATSUI Toshiro
Division of Bioscience and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School of Kyu
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Matsumoto K
Fac. Of Agriculture Graduate School Kyushu Univ.
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Toshiro Matsui
Faculty Of Agriculture Graduate School Kyushu University
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Matsumoto K
Gifu International Institute Of Biotechnology
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Matsui Toshiro
Division Of Bioresources And Biosciences Faculty Of Agriculture Graduate School Of Kyushu University
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Matsumoto Kiyoshi
Division Of Food Biotechnology Department Of Bioscience And Biotechnology Faculty Of Agriculture Gra
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Matsumoto K
Central Research Laboratory Hitachi Ltd.
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Matsumoto Kiyoshi
Division of Bioscience and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School of Kyushu University
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