Electrochemical Measurement of Yeast Cell Density and Vitality Using 2,3,5-Trimethyll, 4-benzoquinone and Effect of Ethanol on the Metabolism of Quinone by Yeast Cells
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概要
- 論文の詳細を見る
An electrochemical measurement of yeast cell density and vitality was developed using 2,3,5-trimethyl-1,4-benzoquinone. 2,3,5-Trimethyl-1,4-benzoquinone was reduced most effectively to the corresponding hydroquinone by yeast cells. The hydroquinone produced was detected amperometrically on the glassy carbon electrode. Ethanol was essential for the effective metabolism of quinones by yeast cells. The maximum oxidative current was obtained at the ethanol concentration of 8–10% regardless of the strain of yeast. The promotion of the metabolism of quinones was thought to be based on the increase of plasma membrane permeability by ethanol molecules. A linear relationship between the oxidative current and cell density of yeast was obtained in the range of 6.4103–1.6106 cells/ml assay solution. During the cultivation of yeast cells the oxidative current showed an almost parallel change with that of glucose in yeast growth and fermentation, suggesting that the oxidative current change reflected the vitality of yeast cells.
- 社団法人 日本食品科学工学会の論文
- 2003-08-01
著者
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UKEDA Hiroyuki
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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MATSUMOTO Kiyoshi
Division of Toxicology, National Institute of Hygienic Sciences
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Ide Seiji
Biotechnology And Food Research Institute Fukuoka Industrial Technology Center
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Ukeda H
Kochi Univ. Nankoku Jpn
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Ukeda Hiroyuki
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Oba Takahiro
Biotechnology And Food Research Institute Fukuoka Industrial Technology Center
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Tsukatani T
Biotechnology And Food Research Institute Fukuoka Industrial Technology Center
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Matsumoto Kiyoshi
Institute Of Food Biotechnology Department Of Bioscience And Biotechnology Division Of Bioresource A
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TSUKATANI Takayuki
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
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Matsumoto Kiyoshi
Division Of Food Biotechnology Department Of Bioscience And Biotechnology Faculty Of Agriculture Gra
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Matsumoto Kiyoshi
Division of Bioscience and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School of Kyushu University
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OBA Takahiro
Biotechnology and Food Research Institute
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IDE Seiji
Biotechnology and Food Research Institute
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