Isolation of a high malic and low acetic acid-producing sake yeast Saccharomyces cerevisiae strain screened from respiratory inhibitor 2,4-dinitrophenol (DNP)-resistant strains(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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We isolated 2,4-dinitrophenol (DNP)-resistant sake yeast strains by UV mutagenesis. Among the DNP-resistant mutants, we focused on strains exhibiting high malic acid and low acetic acid production. The improved organic acid composition is unlikely to be under the control of enzyme activities related to malic and acetic acid synthesis pathways. Instead, low mitochondrial activity was observed in DNP-resistant mutants, indicating that the excess pyruvic acid generated during glycolysis is not metabolized in the mitochondria but converted to malic acid in the cytosol. In addition, the NADH/NAD^+ ratio of the DNP-resistant strains was higher than that of the parental strain K901. These results suggest that the increased NADH/NAD^+ ratio together with the low mitochondrial activity alter the organic acid composition because malic acid synthesis requires NADH, while acetic acid uses NAD^+.
著者
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Kusumoto Kenichi
Biotechnology And Food Research Institute Fukuoka Industrial Technology Center
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Kadokura Toshimori
Department Of Fermentation Science And Technology Faculty Of Applied Biosciences Tokyo University Of Agriculture
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Nakayama Shunichi
Department Of Fermentation Science And Technology Faculty Of Applied Biosciences Tokyo University Of Agriculture
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Nakazato Atsumi
Department Of Fermentation Science And Technology Faculty Of Applied Biosciences Tokyo University Of Agriculture
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OBA Takahiro
Biotechnology and Food Research Institute
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Kadokura Toshimori
Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture
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Kosugi Shingo
Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture
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Kiyoshi Keiji
Department of Fermentation Science and Technology, Faculty of Applied Bio-science, Tokyo University of Agriculture
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