Spectrophotometric Assay for Superoxide Dismutase Based on the Reduction of Highly Water-soluble Tetrazolium Salts by Xanthine-Xanthine Oxidase
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概要
- 論文の詳細を見る
Two novel highly water-soluble tetrazolium salts, WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) and WST-8 (4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate sodium salt) were applied to the assay of superoxide dismutase (SOD). The superoxide anion generated by xanthine/xanthine oxidase (XO) reduced WST-1 and WST-8 to water-soluble formazans which exhibited absorbance maxima at 438 and 460 nm, respectively. The rates of reduction were linearly related to the XO activity, and reduction was inhibited by SOD. Complete inhibition by SOD of the reduction of both WST-1 and WST-8 was achieved, suggesting that these WSTs were not reduced with XO. WST-1 was found more useful than WST-8 because it had shown higher sensitivity which was apparently not dependent on the assay pH value in the range pH 8.0-10.2. These properties of WST-1 are ideal for the spectrophotometric assay of SOD in an aqueous system.
- 社団法人日本農芸化学会の論文
- 1999-03-23
著者
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SAWAMURA Masayoshi
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Sawamura M
Kochi Univ. Nankoku Jpn
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Sawamura M
Kochi Univ. Kochi Jpn
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Sawamura Masayoshi
Department Of Agricultural Chemistry Faculty F Agriculture Kochi University
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UKEDA Hiroyuki
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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MAEDA Susumu
Department of Entomology, University of California
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Sawamura M
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Maeda Susumu
Department Of Entomology University Of California
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Ukeda H
Kochi Univ. Nankoku Jpn
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Ukeda Hiroyuki
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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KAWANA Daisuke
Department of Bioresources Science, Faculty of Agriculture, Kochi University
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Kawana Daisuke
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Maeda S
Department Of Bioresources Science Faculty Of Agriculture Kochi University
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Maeda Susumu
Department Of Agronomy Faculty Of Agriculture Tottori University
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