Isolation and Characterization of Nuclear Polyhedrosis Viruses from the Beet Armyworm Spodoptera exigua (Lepidoptera: Noctuidae) Found in Shiga, Japan
スポンサーリンク
概要
- 論文の詳細を見る
Eleven larvae of the beet armyworm, Spodoptera exigua, showing symptoms of nuclear polyhedrosis virus (NPV) infection were collected in Shiga Prefecture, Japan. NPVs isolated from each larva were divided into two groups. Group 1 isolates produced cuboidal polyhedra, while group 2 isolates produced icosahedral polyhedra. The restriction endonuclease patterns of viral DNA within the same group were identical. The restriction endonuclease patterns of group 1 were similar to those of Autographa californica NPV (AcNPV), whereas the patterns f group 2 were similar to S. exigua NPV isolated in California. SDS-polyacrylamide gel electrophoresis analysis of polyhedra also indicated that SeNPVs differed between the two groups. Group 1 SeNPVs could infect the cell lines SF-21 and TN-368 and group 2 SeNPVs could not. Furthermore, group 1 SeNPVs were able to infect Spodoptera litura, Plutella xylostella, and S. exigua larvae but not Bombyx mori larvae. Group 2 SeNPVs could infect S. exigua larvae, but not S. litura, P. xylostella, or B. mori larvae. The LD_<50> of SeNPV #^5 (group 1) and SeNPV #^1 (group 2) were 58 and 4.8 polyhedra per larva, respectively. These results indicated that the SeNPVs isolated in Shiga Prefecture were composed of two genetically and phenotypically distinct groups.
- 日本応用動物昆虫学会の論文
- 1994-02-25
著者
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MAEDA Susumu
Department of Entomology, University of California
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Maeda S
Department Of Entomology University Of California
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Maeda Susumu
Department Of Entomology University Of California
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Kondo Atsushi
Shiga Plant Protection Office
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Yamamoto Masanori
Shiga Agricultural Experiment Station
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TAKASHI Shousuke
Shiga Agricultural Experiment Station
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Maeda Susumu
Department Of Agronomy Faculty Of Agriculture Tottori University
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Kondo Atsushi
Shiga Agricultural Experiment Station
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- Isolation and Characterization of Nuclear Polyhedrosis Viruses from the Beet Armyworm Spodoptera exigua (Lepidoptera: Noctuidae) Found in Shiga, Japan