Expression of an enhancin gene from the Trichoplusia ni granulosis virus confers resistance to lepidopterous insect pests to rice
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概要
- 論文の詳細を見る
Transgenic plants that produce anti-insect substances are vital in improving crop yields and in reducing the environmental risks of chemical insecticides. Enhancin is a metalloprotease produced in occlusion bodies of the Trichoplusia ni granulovirus (TnGV). It is a key substance that enhances infection of the nucleopolyhedrovirus in lepidopteran insects. Rice (Oryza sativa L. cv. Nipponbare) protoplasts were cotransformed with pREXΦVEF and pLTHyg, which respectively bear the chimeric enhancin gene and the hygromycin-resistance gene. Hygromycin-resistant regeneration plants were examined by genomic polymerase chain reaction and genomic Southern and northern blotting analyses to confirm the presence and expression of the enhancin gene. Fourteen transgenic plant lines harboring the enhancin gene were obtained, and stable inheritance and expression of the enhancin gene were confirmed in the second, third, and fourth plant generations. Feeding Spodoptera exigua larvae leaves of enhancin-expressing rice plants in the presence of S. exigua nucleopolyhedrovirus occlusion bodies enhanced infection of the virus. Further, the development of Pseudaletia separata, S. exigua, and S. litura, none of which are host insects of TnGV, was inhibited when these larvae were fed enhancin-expressing rice leaves. This indicates that expression of the enhancin gene confers resistance to lepidopteran insect pests in rice.
- 日本植物細胞分子生物学会の論文
- 2006-03-01
著者
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Dohi Koji
Ishikawa Prefectural Univ. Ishikawa Jpn
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Dohi Koji
Ishikawa Prefectural University
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Mise Kazuyuki
Graduate School Of Agriculture Kyoto University
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Kaido M
Graduate School Of Agriculture Kyoto University
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MORI Mari
Shiga Prefecture Agricultural Technology Promotion Center
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KITAMURA Harushige
Shiga Prefecture Agricultural Technology Promotion Center
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KONDO Atsushi
Shiga Prefecture Government
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MORI Masashi
Ishikawa Prefectural University
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KAIDO Masanori
Graduate School of Agriculture, Kyoto University
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SHIMOJYO Eiichi
Faculty of Textile Science, Kyoto Institute of Technology
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HASHIMOTO Yoshifumi
Faculty of Textile Science, Kyoto Institute of Technology
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Mori M
Shiga Prefecture Agricultural Technology Promotion Center
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Kaido Masanori
Graduate School Of Agriculture Kyoto University
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Shimojo Ei-ichi
Faculty Of Textile Science Kyoto Institute Of Technology
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Hashimoto Y
Faculty Of Textile Science Kyoto Institute Of Technology
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Mori Masashi
Ishikawa Prefectural Univ. Ishikawa Jpn
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Hashimoto Yoshifumi
Faculty Of Textile Science Kyoto Institute Of Technology
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Kondo Atsushi
Shiga Plant Protection Office
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Kondo Atsushi
Shiga Agricultural Experiment Station
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