Rapid Quantification Methods for Genetically Modified Maize Contents Using Genomic DNAs Pretreated by Sonication and Restriction Endonuclease Digestion for a Capillary-Type Real-Time PCR System with a Plasmid Reference Standard
スポンサーリンク
概要
- 論文の詳細を見る
For rough quantitative analysis of genetically modified maize contents, rapid methods for measurement of the copy numbers of the cauliflower mosaic virus 35S promoter region (P35S) and MON810 construct-specific gene (MON810) using a combination of a capillary-type real-time PCR system with a plasmid DNA were established. To reduce the characteristic differences between the plasmid DNA and genomic DNA, we showed that pretreatment of the extracted genomic DNA by a combination of sonication and restriction endonuclease digestion before measurement is effective. The accuracy and reproducibility of this method for MON810 content (%) at a level of 5.0% MON810 mixed samples were within a range from 4.26 to 5.11% in the P35S copy number quantification. These methods should prove to be a useful tool to roughly quantify GM maize content.
- 社団法人 日本農芸化学会の論文
- 2006-12-23
著者
-
Maitani Tamio
国立医薬品食品衛生研究所
-
Maitani Tamio
National Institute of Health Sciences
-
AKIYAMA Hiroshi
National Institute of Health Sciences
-
Maitani Tamio
Division of Foods, National Institute of Health Sciences
-
HINO Akihiro
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
-
WATANABE Takahiro
National Institute of Health Sciences
-
Kondo Kazunari
National Inst. Of Health Sciences
-
Maitani T
Division Of Foods National Institute Of Health Sciences
-
TOYOTA Akie
Hiroshima Prefectural Institute of Public Health and Environment
-
SUGIMURA Mitsunori
Hiroshima Prefectural Institute of Public Health and Environment
-
SAKATA Kozue
National Institute of Health Sciences
-
SHIRAMASA Yuko
National Institute of Health Sciences
-
KITTA Kazumi
National Food Research Institute
-
ESAKA Muneharu
Graduate School of Biosphere Sciences, Hiroshima University
-
Sasaki K
Hokkaido Univ. Sapporo Jpn
-
Kitta Kazumi
National Food Res. Inst.
-
Machiguchi Takahisa
Faculty Of Science Saitama University
-
Maitani Tamio
National Institute For Environmental Studies
-
Toyota Akie
Hiroshima Prefectural Institute For Public Health And Environment Science
-
米谷 民雄
Division Of Foods National Institute Of Health Sciences
-
Hino Akihiro
National Food Research Institute Ministry Of Agriculture Forestry And Fisheries
-
Sakata Kozue
National Inst. Health Sci. Tokyo Jpn
-
Akiyama H
Real Estate Science Graduate School Of Science And Technology Nihon University
-
Esaka Muneharu
Graduate School Of Biosphere Science Hiroshima University
-
Akiyama Hiroshi
Division Of Novel Foods And Immunochemistry National Institute Of Health Science
-
Akiyama Hiroshi
National Food Research Institute National Agriculture And Food Research Organization
-
HINO Akihiro
National Food Research Institute (NFRI), National Agriculture and Food Research Organization (NARO)
関連論文
- 耐酸性コチニール(Acid-Stable Carmine)の化学構造
- スギヒラタケに含まれる特徴的な長鎖脂肪酸について
- マウス膝窩リンパ節測定法による天然着色料(コチニール色素、ラック色素および色素本体化合物)の抗原性評価の試み
- Speciation of Aluminium in Human Serum Investigated by HPLC/High Resolution Inductively Coupled Plasma Mass Spectrometry (HR-ICP-MS) : Effects of Sialic Acid Residues of the Carbohydrate Chain on the Binding Affinity of Aluminium for Transferrin
- スルメイカおよびサンマの可食部位別におけるダイオキシン類汚染レベル
- ヘッドスペースGC/MS法によるベビーフードおよび粉ミルク中のフランの迅速改良定量法
- 13C 標識アクリルアミドを内標準に用いたGC/MSによる食品中アクリルアミドの分析
- Conjugated Ketonic Fatty Acids from Pleurocybella porrigens
- P-236 CONJUGATED LONG-CHAIN FATTY ACIDS OF PLEUROCYBELLA PORRIGENS
- Multiplex PCR法を用いた組換えトウモロコシ5系統からの組換え遺伝子の検知法
- Duplex PCR法を用いた遺伝子組換えパパイヤ改良検知法
- 組織化学的手法を用いた遺伝子組換えパパイヤ(55-1)検知法の確立
- カラムスイッチングLC/MSによる加工食品中のアクリルアミドの分析
- Measurement of a Genotoxic Hydrazine, Agaritine, and Its Derivatives by HPLC with Fluorescence Derivatization in the Agaricus Mushroom and Its Products(Communications to the Editor)
- Frequency of Mutations of the Transgene, which might Result in the Loss of the Glyphosate-Tolerant Phenotype, was Lowered in Roundup Ready^[○!R] Soybeans
- Constituents in Watercress : Inhibitors of Histamine Release from RBL-2H3 Cells Induced by Antigen Stimulation
- The Effect of Feeding Carrots on Immunoglobulin E Production and Anaphylactic Response in Mice
- Structural Determination of Unknown Subsidiary Colors in Commercial Food Red Nos. 2 and 102
- Glutathione Enhanced Anthraquinone Production in Adventitious Root Cultures of Rubia tinctorum L
- IDENTIFICATION OF MAJOR PIGMENTS CONTAINING D-AMINO ACID UNITS IN COMMERCIAL MONASCUS PIGMENTS
- Structural Determination of Unknown Subsidiary Colors in Food Yellow No. 5(Sunset Yellow FCF)
- High-performance Liquid Chromatographic Analyses of Capsaicinoids and Their Phenolic Intermediates in Capsicum annuum to Characterize Their Biosynthetic Status
- 酵素分解ハトムギ抽出物の主要成分の分析
- A Cembrane-type Diterpene from Flue-cured Burley Tobacco (Nicotiana tabacum L. ) leaves
- Two New Alkaloids from Cigarette Smoke Condensate
- HPLC-ICP STUDY OF VARIOUS METALS ADDED IN VITRO TO MOUSE SERUM
- Dioxin Concentrations in Commercial Health Tea Materials in Japan
- A DNA Extraction Method Using a Silica-base Resin Type Kit for the Detection of Genetically Modified Papaya
- Rapid Quantification Methods for Genetically Modified Maize Contents Using Genomic DNAs Pretreated by Sonication and Restriction Endonuclease Digestion for a Capillary-Type Real-Time PCR System with a Plasmid Reference Standard
- Sugihiratake Mushroom (Angel's Wing Mushroom)-Induced Cryptogenic Encephalopathy may Involve Vitamin D Analogues(Pharmacognosy)
- A Detection Method of CryIAc Protein for Identifying Genetically Modified Rice Using the Lateral Flow Strip Assay
- Quantification of Genetically Modified Soybeans Using a Combination of a Capillary-Type Real-Time PCR System and a Plasmid Reference Standard
- Analysis of agaritine in mushrooms and in agaritine-administered mice using liquid chromatography-tandem mass spectrometry
- Effect of Oral Administration of CpG ODN-OVA on WBB6F1-W/W^v Mice
- Determination of Cyanide and Thiocyanate in Sugihiratake Mushroom Using HPLC Method with Fluorometric Detection
- The Hyperresponsiveness of W/W^v Mice to Oral Sensitization Is Associated with a Decrease in TCRγδ-T Cells(Biochemistry/Molecular Biology)
- New Qualitative Detection Methods of Genetically Modified Potatoes(Highlighted paper selected by Editor-in-chief)(Analytical Biochemistry)
- The Feeding of β-Carotene Down-Regulates Serum IgE Levels and Inhibits the Type I Allergic Response in Mice(Highlighted paper selected By Editor-in-chief)(Analytical Biochemistry)
- 緑葉野菜中のダイオキシン類測定における前処理法の改良と多層シリカゲルカラムの充てん法の比較
- Evaluation of a Nitric Acid-based Partial-digestion Method for Selective Determination of Inorganic Arsenic in Rice
- Nitric Acid-based Partial-digestion Method for Selective Determination of Inorganic Arsenic in Hijiki and Application to Soaked Hijiki
- Naringin Glycosides α-Glucosylated on Ring B Found in the Natural Food Additive, Enzymatically Modified Naringin
- 酵素処理イソクエルシトリン及び酵素処理ルチン(抽出物)の成分分析
- 天然甘味料テンリョウチャ抽出物の主甘味成分ルブソシド及び関連成分の分析
- Polyphenolic Constituents from the Leaves of Rubus suavissimus
- In Vitro Effects of Synthetic Dyes on the Syntheses of Prostaglandin E_2 (PGE_2) and 5-Hydroxyeicosatetraenoic Acid (5-HETE)
- RENAL CRITICAL CONCENTRATION OF CADMIUM IN THE ACUTE NEPHROPATHY BY CADMIUM INJECTED WITH CYSTEINE
- 日本におけるファーストフード中のダイオキシン類レベルとその同族体分布
- 食品中のダイオキシン類測定の前処理における硫酸処理法と多層シリカゲルカラムクロマトグラフ法の比較
- Optical Detection of Specific Genes for Genetically Modified Soybean and Maize Using Multiplex PCR Coupled with Primer Extension on a Plastic Plate
- Real-Time PCR Method Using Capturing Oligo-Immobilized PCR Tubes to Determine the Specific Gene for Soybean and Genetically Modified Soybean in Food Matrices
- 日本における市販生鮮および冷凍野菜中のPCDDs,PCDFsおよびコプラナーPCBsレベルと各同族体分布
- プレパック多層シリカゲルカラムを用いた前処理による食品中のダイオキシン類分析
- Detection of Walnut Residues in Processed Foods by Polymerase Chain Reaction
- Novel Method to Detect a Construct-Specific Sequence of the Acetolactate Synthase Gene in Genetically-Modified Flax CDC Triffid (FP967)
- A Screening Method for the Detection of the 35S Promoter and the Nopaline Synthase Terminator in Genetically Modified Organisms in a Real-Time Multiplex Polymerase Chain Reaction Using High-Resolution Melting-Curve Analysis
- Specific Detection of Buckwheat Residues in Processed Foods by Polymerase Chain Reaction
- Specific Detection of Soybean Residues in Processed Foods by the Polymerase Chain Reaction
- Specific Detection of Wheat Residues in Processed Foods by Polymerase Chain Reaction
- IC/ICP-MSによるパン中臭素酸カリウムの特異的分析法
- Detection of Hydrocarbons in Irradiated Foods
- STRAIN DIFFERENCES IN CHANGES OF ESSENTIAL METAL LEVELS IN TESTES AND OTHER ORGANS OF MICE AFTER CADMIUM ADMINISTRATION
- COMPARISON OF CHEMICAL PROPERTIES OF METALLOTHIONEIN-I AND -II
- Comparison of metal-metal interaction after co-administration of arsenic and selenium or arsenic and tellurium into mice
- Six Diacylated Anthocyanins from the Storage Roots of Purple Sweet Potato, Ipomoea batatas
- Antiallergic Effect of Apple Polyphenols on the Allergic Model Mouse
- Anti-allergic effect of apple polyphenol on patients with atopic dermatitis : A pilot study
- Spontaneous Rupture of the Stomach in Preschool Age Children:A Report of Two Cases
- Giant Mediastinal Lymphangioma in a Neonate : Report of a Case
- 全国の行政検査結果(1998年度)を基にした食品中の防かび剤の濃度実態及び摂取量の推定
- ESR Method for the Detection of Irradiated Unboned Meats and Seafood
- Baseline Noise and Measurement Uncertainty in Liquid Chromatography
- 遺伝子組換えとうもろこしCBH351のBNラット並びにB10Aマウスへの90日間混餌投与による免疫系への影響
- LC/MSによる5-Enolpyruvylshikimate-3-phosphate Synthaseの酵素活性の測定
- Inhibitory Effects of Apple Polyphenol on Induced Histamine Release from RBL-2H3 Cells and Rat Mast Cells
- 遺伝子組換え食品導入蛋白質(CP4-EPSPS, Cry1Ab)の熱感受性並びにin vitro 分解性試験
- 遺伝子組換え, 非組換え大豆のマウス, ラットへの混餌投与による免疫系への影響
- トウモロコシからの組換え遺伝子の検知法
- Real-Time Polymerase Chain Reaction Method for Detecting Contamination of Beef by Material from Genetically Engineered Cattle
- QUANTITATIVE EVALUATION OF PASSIVE CUTANEOUS ANAPHYLAXIS (PCA) USING A HAND-HELD SPECTROPHOTOMETER
- Detection of Walnut Residues in Processed Foods by Polymerase Chain Reaction
- Evaluation of Quantitative PCR Methods for Genetically Modified Maize (MON863, NK603, TC1507 and T25)
- Optical Detection of Specific Genes for Genetically Modified Soybean and Maize Using Multiplex PCR Coupled with Primer Extension on a Plastic Plate
- Real-Time PCR Method Using Capturing Oligo-Immobilized PCR Tubes to Determine the Specific Gene for Soybean and Genetically Modified Soybean in Food Matrices
- Specific Detection of Buckwheat Residues in Processed Foods by Polymerase Chain Reaction
- Specific Detection of Soybean Residues in Processed Foods by the Polymerase Chain Reaction
- Specific Detection of Wheat Residues in Processed Foods by Polymerase Chain Reaction
- コ-ヒ-豆及び穀類中のオクラトキシンAの迅速分析法
- Simple HPLC Determination of Aflatoxins B_1, B_2, G_1 and G_2 in Nuts and Corn
- 4-(N,N-dimethylaminosulhonyl)-7-fluoro-2,1,3-benzoxadiazole(DBD-F)を用いたプレカラムHPLC法によるとうもろこし中のフモニシンB1,B2の定量〔英文〕
- Identification and Detection Method for Genetically Modified Papaya Resistant to Papaya Ringspot Virus YK Strain
- Quantification of Pork, Chicken and Beef by Using a Novel Reference Molecule
- A Real-Time Quantitative PCR Detection Method for Pork, Chicken, Beef, Mutton, and Horseflesh in Foods
- FXYD6, a Na,K-ATPase Regulator, Is Expressed in Type II Taste Cells
- Interaction of Some Plant Food Extracts with Aryl Hydrocarbon Receptor Determined by in Vitro Reporter Gene Assay
- Trypanosome alternative oxidase, a potential therapeutic target for sleeping sickness, is conserved among Trypanosoma brucei subspecies
- Quantification of Pork, Chicken and Beef by Using a Novel Reference Molecule
- Specific Detection by the Polymerase Chain Reaction of Potentially Allergenic Salmonid Fish Residues in Processed Foods
- Multiplex Real-Time PCR Assay for Simultaneous Detection of Omphalotus guepiniformis and Lentinula edodes
- Quantitative Determination of Carthamin in Carthamus Red by 1H-NMR Spectroscopy