Novel Method to Detect a Construct-Specific Sequence of the Acetolactate Synthase Gene in Genetically-Modified Flax CDC Triffid (FP967)
スポンサーリンク
概要
- 論文の詳細を見る
During the fall of 2009, a trace of unauthorized genetically modified (GM) flax (Linum usitatissimum L.) line, CDC Triffid, which is resistant to sulfonylurea herbicides, was detected in many countries including Japan. A method to reliably identify the CDC Triffid line was urgently required. We developed a novel construct-specific real-time polymerase chain reaction (PCR) method to identify the mutant acetolactate synthase gene in the CDC Triffid line. We confirmed that the method can detect 0.001% GM flax in DNA mixing solution. The study shows that the developed method is specific, sensitive and reliable way to monitor a trace of CDC Triffid.
著者
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TESHIMA Reiko
National Institute of Health Sciences
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AKIYAMA Hiroshi
National Institute of Health Sciences
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KAWAKAMI HIROSHI
Department of Gastroenterology, Hokkaido University Graduate School of Medicine
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SAKATA Kozue
National Institute of Health Sciences
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KITTA Kazumi
National Food Research Institute
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Nakamura Kosuke
National Institute of Health Sciences
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Yamada Chihiro
Department of Food Science & Nutrition, Kyoritsu Womens University
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Satoh Rie
National Institute of Health Sciences
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Makiyama Daiki
National Institute of Health Sciences
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Mano Junichi
National Food Research Institute
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Satoh Rie
National Inst. Of Health Sciences
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Kitta Kazumi
National Food Res. Inst.
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Teshima Reiko
National Inst. Of Health Sciences
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Sakata Kozue
National Inst. Health Sci. Tokyo Jpn
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Akiyama Hiroshi
National Food Research Institute National Agriculture And Food Research Organization
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Kawakami Hiroshi
Department Of Gastroenterology And Hepatology Hokkaido University
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Yamada Chihiro
Department of Food Science & Nutrition, Kyoritsu Women's University
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Kawakami Hiroshi
Department of Food Science & Nutrition, Kyoritsu Women's University
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Kawakami Hiroshi
Department of Cardiology, Ehime Prefectural Central Hospital
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