Quantification of Pork, Chicken and Beef by Using a Novel Reference Molecule
スポンサーリンク
概要
- 論文の詳細を見る
A standard plasmid was constructed as a novel reference molecule for use in real-time quantitative PCR assays to verify the identity of beef, pork, chicken, mutton, and horseflesh. The plasmid contained a target domain of the cytochrome b (cyt b) gene and an artificial DNA sequence. Primers CO-F and CO-R, and probe CO-P were specifically designed to detect the artificial sequence. The calculated R2 values of the standard curves (103–107 copies per reaction) for the five species ranged between 0.998 and 0.999 in the quantification analysis. The constructed plasmid provides a universal method for measuring the copy number of cyt b DNA in minced meat. This method would be a useful procedure for verifying food labels.
- 社団法人 日本農芸化学会の論文
著者
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AKIYAMA Hiroshi
National Institute of Health Sciences
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Sugita-Konishi Yoshiko
National Institute of Health Sciences
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Sakai Yumiko
Nagahama Institute For Biochemical Science Oriental Yeast Co. Ltd.
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Tanabe Soichi
Graduate School Of Biosphere Sci. Hiroshima Univ.
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Yano Takeo
Nagahama Institute For Biochemical Science Oriental Yeast Co. Ltd.
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Yano Takeo
Biochemical Production And Development Center Nagahama Branch Oriental Yeast Co. Ltd.
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Kotoura Satoshi
Central Research Institute Marudai Food Co. Ltd.
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SAKAI Yumiko
Biochemical Production and Development Center, Nagahama Branch, Oriental Yeast Co., Ltd.
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KURIHARA Takashi
Biochemical Production and Development Center, Nagahama Branch, Oriental Yeast Co., Ltd.
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UCHIDA Kouji
Biochemical Production and Development Center, Nagahama Branch, Oriental Yeast Co., Ltd.
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MIAKE Kiyotaka
Central Research Institute, Marudai Food Co., Ltd.
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