Sensitivity of Hep G2 Cells to Bacillus cereus Emetic Toxin
スポンサーリンク
概要
- 論文の詳細を見る
We herein examined the sensitivity of Hep G2 human hepatoma cells to Bacillus cereus emetic toxin. Hep G2 cells were treated with the emetic toxin, and the cell shape was observed. The same experiments were performed for comparison purposes, using HEp-2 cells, which are currently used by most laboratories for a bioassay of the emetic toxin. Hep G2 cells showed clearer vacuolation in the cytosol within 2 hr and required a shorter incubation period than HEp-2 cells (10 hr). The number of vacuoles in the Hep G2 cells was greater, and the size of the vacuoles was larger than those observed in HEp-2 cells. The minimal concentration of the emetic toxin required to induce the vacuolation of Hep G2 cells was 0.04 ng/ml. The concentration for the HEp-2 cells was 1 ng/ml. These findings indicate that Hep G2 cells show higher sensitivity to the emetic toxin. Hep G2 cells may be superior to the currently used HEp-2 cells for the bioassay of the emetic toxin.
著者
-
Agata Norio
Nagoya City Public Health Res. Inst. 1-11 Hagiyama-cho Mizuho-ku Nagoya 467-8615 Jpn
-
Sugita-Konishi Yoshiko
National Institute of Health Sciences
-
Sugita-konishi Yoshiko
National Inst. Of Health Sciences
-
KAMATA Yoichi
National Institute of Health Sciences
-
KANNO Shinji
National Institute of Health Sciences
-
MIZUTANI Noriko
National Institute of Health Sciences
-
KAWAKAMI Hiroshi
Kyoritsu Women’s University
-
SUGIYAMA Kei-ichi
National Institute of Health Sciences
-
KAWAKAMI Hiroshi
Kyoritsu Women's University
関連論文
- A survey of the occurrence of Fusarium mycotoxins in biscuits in Japan by using LC/MS
- Determination of Cyanide and Thiocyanate in Sugihiratake Mushroom Using HPLC Method with Fluorometric Detection
- Phylogenetic Analysis of Bacillus cereus Isolates from Severe Systemic Infections Using Multilocus Sequence Typing Scheme
- Quantitative Analysis of Cereulide, an Emetic Toxin of Bacillus cereus, by Using Rat Liver Mitochondria
- Growth Conditions of and Emetic Toxin Production by Bacillus cereus in a Defined Medium with Amino Acids
- The Effect of Naringenin on the Fate and Disposition of Deoxynivalenol in Piglets
- Effect of Cooking Process on the Deoxynivalenol Content and Its Subsequent Cytotoxicity in Wheat Products
- Pharmacokinetic Characteristics of Labeled-Nivalenoland -FusarenonX in Mice.(GENERAL SESSION BY POSTER PRESENTATION)(DRUG METABOLISM)
- Detection of Walnut Residues in Processed Foods by Polymerase Chain Reaction
- Quantification of Pork, Chicken and Beef by Using a Novel Reference Molecule
- Quantification of Pork, Chicken and Beef by Using a Novel Reference Molecule
- Specific Detection by the Polymerase Chain Reaction of Potentially Allergenic Salmonid Fish Residues in Processed Foods
- Sensitivity of Hep G2 Cells to Bacillus cereus Emetic Toxin